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Millipore This Rat Monoclonal Antibody Anti-Cd115 (C-Fms) (Mouse), Pe, Clone Afs98 Validated For Use In Western Blotting & Flow Cytometry For The Detection Of Cd115 (C-Fms). - Mill (Additional S&H or Hazmat Fees May Apply)
The AFS98 antibody is specific for mouse CD115, also known as Colony-Stimulating Factor-1 Receptor (CSF-1R), a 145 kDa receptor from the PDGF receptor family. Receptor activation by the ligands IL-34 or CSF-1 (M-CSF) occurs via homodimerization of CD115 and subsequent tyrosine phosphorylation and ubiquitination of intracellular domains. CD115 signaling promotes differentiation of myeloid precursors, as well as the continued regulation of proliferation, survival and function of mononuclear phagocytes, dendritic cells and osteoclasts. While IL-34 and CSF-1 may induce similar cellular responses, they are differentially expressed and as such exert complimentary actions via CD115.The AFS98 antibody may be used for identification of myeloid lineage cells by flow cytometry, and is commonly used for in vivo or in vitro neutralization of CSF-1 Receptor.
Product Information
Format
PE
Presentation
Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
This rat monoclonal antibody Anti-CD115 (c-fms) (mouse), PE, clone AFS98 validated for use in western blotting & Flow Cytometry for the detection of CD115 (c-fms).
Key Applications
Flow Cytometry
Western Blotting
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
AFS98
Concentration
0.2 mg/mL
Host
Rat
Isotype
IgG2aκ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
Csf1r
Csfmr
Fms
Purification Method
Size Exclusion Chromatography
UniProt Number
P09581
Product Usage Statements
Quality Assurance
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.125 µg of this antibody detected CD115 (c-fms) in one million C57Bl/6 peripheral blood cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
