| Description | |
|---|---|
| Catalogue Number | MABF824-25UL |
| Description | Anti-REG3A Antibody, clone 8B11.1 |
| Alternate Names |
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| Background Information | Regenerating islet-derived protein 3-alpha (UniProt: Q06141; also known as REG-3-alpha, Hepatointestinal pancreatic protein, HIP/PAP, Human proislet peptide, Pancreatitis-associated protein 1, Regenerating islet-derived protein III-alpha, Reg III-alpha) is encoded by the REG3A (also known as PAP, PAP1) gene (Gene ID: 5068) in human. REG3-alpha is a member of the C-type lectin super family that exhibits bactericidal activity against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. It is a secretory protein that appears in pancreatic juice after induction of pancreatic inflammation and is shown to be highly induced during acute pancreatitis. In normal healthy pancreas, very low to undetectable expression is seen. It is also involved in hepatic and pancreatic regeneration. REG3-alpha is synthesized with a signal peptide (aa 1-26) and a propeptide (aa 27-37) that are cleaved off in the mature protein. Proteolytic processing by trypsin is shown to remove the inhibitory propeptide and this step is essential for peptidoglycan binding and antibacterial activity. REG3-alpha contain a C-type lectin domain (aa 47-172) and an EPN (Glu-Pro-Asn) motif (aa 114-116), The EPN motif is essential for recognition of the peptidoglycan carbohydrate backbone and for efficient bacterial killing with Glu114 playing a key role in peptidoglycan binding and bactericidal activity. |
| Product Information | |
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| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-REG3A, clone 8B11.1, Cat. No. MABF824, is a mouse monoclonal antibody that detects Regenerating islet-derived protein 3-alpha (REG3A) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
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| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected REG3A in human small intestine and human stomach tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fragment corresponding to 128 amino acids from the internal region of human Regenerating islet-derived protein 3-alpha. |
| Clone | 8B11.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 8B11.1 is a mouse monoclonal antibody that detects human Regenerating islet-derived protein 3-alpha. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed; 19.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in human small intestine tissue lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Regenerating islet-derived protein 3-alpha in Human small intestine tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 µL |