Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IP | AHm | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF930-25UG |
| Description | Anti-TCR Vγ1.1 Antibody, clone 2.11 |
| Alternate Names |
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| Background Information | T cell receptor (TCR) is a single-pass membrane protein that associates with CD3 to form a CD3/TCR complex. It can be a heterodimer of either alpha and beta chains or gamma and delta chains. CD3/RCR complex plays major role in antigen recognition and activation of T cells. The V gamma 1 gene almost exclusively rearranges to the J gamma 4-C gamma 4 gene. V-gamma 1- J gamma 4-C gamma 4 expressing cells form a major population of gamma/delta T cells in thymus and peripheral lymphoid organs in adult mice. However, during early development they are present in lower numbers. This antibody (clone 2.11) recognizes the V gamma 1.1 chain of the heterodimeric gamma/delta T-cell receptor, but does react with TCR alpha/beta bearing cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-TCR V 1.1, clone 2.11, Cat. No. MABF930, is an armenian hamster monoclonal antibody that detects T-cell receptor gamma chain C region C10.5. and has been tested for use Flow Cytometry, and Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected TCR V gamma 1.1 in Flow Cytometry applications (Pereira, P., et. al. (1995). J Exp Med. 182(6):1921-30; Pereira, P., et. al. (2004). J Immunol. 173(5):3261-70). Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected TCR V gamma 1.1 in Lymphocytes isolated from TcrdH2BeGFP reporter mice (Courtesy of Dr rer. nat. Inga Sandrock, Hannover Medical School, Germany). Immunoprecipitation Analysis: A representative lot detected TCR V gamma 1.1 in Immunoprecipitation applications (Pereira, P., et. al. (1995). J Exp Med. 182(6):1921-30). |
| Biological Information | |
|---|---|
| Immunogen | Irradiated TCR gamma/delta expressing T3 .13.1 hybridoma cells. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Armenian Hamster |
| Specificity | Clone 2.11 detects T-cell receptor gamma chain C region C10.5 in lymphocytes. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 19.10 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |