Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF934-25UL |
| Description | Anti-phospho-SAMHD1 (Thr592) Antibody, clone T592P |
| Alternate Names |
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| Background Information | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (UniProt: Q9Y3Z3; also known as dNTPase, Dendritic cell-derived IFNG-induced protein, DCIP, Monocyte protein 5, MOP-5, SAM domain and HD domain-containing protein 1) is encoded by the SAMHD1 (also known as MPOS) gene (Gene ID: 25939) in human. SAMHD1 is a nuclear protein that functions as a host restriction nuclease that blocks early-stage virus replication in dendritic and other myeloid cells. It suppresses LINE-1 retrotransposon activity and may function by reducing the cellular dNTP to such low levels that prevents retroviral reverse transcription. SAMHD1 is also reported to play a role in mediating pro-inflammatory responses to TNF-alpha signaling. It is expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes, however, no expression is seen in brain and thymus tissue. Its expression is shown to be upregulated in TNF-alpha treated fibroblasts. SAMHD1 is allosterically stimulated by dGTP, which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Four isoforms of SAMHD1 have been described that are produced by alternate splicing. Mutations in SAMHD1 gene are reported to be involved in the pathogenesis of Chilblain lupus 2, a cutaneous form of lupus erythematosus and in Aicardi-Goutieres syndrome 5 that is characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-phospho-SAMHD1 (Thr592), clone T592P, Cat. No. MABF934 is a mouse monoclonal antibody that detects SAMHD1 and has been tested for use in Flow Cytometry and Western Blotting. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected phospho-SAMHD1 (Thr592) in Flow Cytometry applications (Coiras, M., et. Al. (2016). Cell Rep. 14(9):2100-7). Western Blotting Analysis: A representative lot detected phospho-SAMHD1 (Thr592) in Western Blotting applications (Cribier, A., et. Al. (2013). Cell Rep. 3(4):1036-43; Calantone, N., et. Al. (2014). Immunity. 41(3):493-502; Coiras, M., et. Al. (2016). Cell Rep. 14(9):2100-7). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 11 amino acids surrounding phosphoryalted Thr592 from the C-terminal region of huamn SAMHD1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone T592P detects Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 in human cells. It targets an epitope with in 11 amino acids surrounding the phosphorylated Thr592 from the C-terminal region. |
| Isotype | IgG2a |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~72 kDa observed; 72.20 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in CD4+ T lymphocytes (activated with PMA/IL-2) lysates. Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-SAMHD1 (Thr592) in CD4+ T lymphocytes (activated with PMA/IL-2) lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |