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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN109 |
| Description | Anti-Synaptotagmin-4 Antibody, clone 10C11.1 |
| Alternate Names |
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| Background Information | Synaptotagmin-4 (UniProt Q9H2B2; also known as Synaptotagmin IV, SytIV) is encoded by the SYT4 (also known as KIAA1342) gene (Gene ID 6860) in human. The mammalian synaptotagmin family is comprised of 17 members. While most of them are predominantly function in the cetnral nervous system (CNS), some are involved in the vesicular functions of endocrine cells and glucose-transport metabolic cells. Synaptotagmin-4 is an inducible member detectable only in the brain and the neuroendocrine system. Unlike other family members, synaptotagmin-4 does not bind calcium and does not medidate calcium-induced exocytosis. Instead, synaptotagmin-4 is shown to play a neuroendocrine role in inhibiting exocytotic activities in the posterior pituitary and in cultured PC12 cells. Synaptotagmin-4 is expressed most abundantly in oxytocin neurons of the hypothalamus, where it negatively regulates oxytocin exocytosis. Dietary obesity is associated with increased vesicle binding of synaptotagmin-4 and decreased oxytocin release. Normalizing oxytocin release via Synaptotagmin-4 inhibition prevents against dietary obesity under chronic nutritional excess. Human synaptotagmin-4 consists of a vesiclular domain (a.a. 1-16), a transmembrane segment (a.a. 17-37),and a large cytoplasmic domain (a.a. 38-425). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Synaptotagmin-4 Antibody, clone 10C11.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Synaptotagmin-4. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synaptotagmin-4 in human kidney, skeletal muscle, and pancreas tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human Synaptotagmin-4 cytoplasmic domain fragment. |
| Epitope | Cytoplasmic domain. |
| Clone | 10C11.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 10C11.1 targets a cytoplasmic epitope present in both human spliced isoforms reported by UniProt (Q9H2B2). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~48 kDa observed. 47.96/46.06 kDa (human isoform 1/2) and 47.66 kDa (mouse) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse cerebellum tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptotagmin-4 in 10 µg of mouse cerebellum tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |