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Millipore Detect Limk1 Using This Anti-Limk1 Antibody, Clone 6c3.1 Validated For Use In Western Blotting & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
LIM Kinase 1 (LIMK1) is a protein kinase that plays an important role in cellular functions like cell motility and growth cone actin dynamics. LIMK1 contains one PDZ domain and two LIM domains which mediate protein to protein inteactions. It is widely expressed in the adult and fetal nervous system with the highest levels being found in the cerebral cortex. Mutations in LIMK1 have been associated with cardiovascular and musculo-skeletal abnormalities.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Limk1 using this Anti-Limk1 Antibody, clone 6C3.1 validated for use in western blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Limk1 in human thalamus and human cerebral cortex tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human Limk1.
Clone
6C3.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002305
Gene Symbol
LIMK1
LIMK
Purification Method
Protein G Purified
UniProt Number
P53667
Molecular Weight
~72 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human cortex tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Limk1 in 10 µg of human cortex tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.