| Replacement Information | |
|---|---|
| Replacement Information | MAB302 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1182 |
| Description | Anti-Glutamine Synthetase Antibody, clone 11A3.1 |
| Alternate Names |
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| Background Information | Glutamine synthetase (EC 4.1.1.15, EC 6.3.1.2; UniProt P09606; also known as Glutamate-ammonia ligase, Glutamate decarboxylase, GS) is encoded by the Glul (also known as Glns) gene (Gene ID 24957) in rat species. Glutamine synthetase (GS) plays an essential role in nitrogen metabolism by catalyzing ATP-dependent condensation of glutamate and ammonia to form glutamine. Three classes of GS exist, class I enzymes (GSI) are specific to prokaryotes and exist as oligomers of 12 identical subunits, class II enzymes (GSII) exist as decamer of identical subunits in eukaryotes and in some bacteria, while class III enzymes (GSIII) function as a double-ringed dodecamer of identical chains found in bacteroides fragilis and in butyrivibrio fibrisolvens. GS is present predominantly in the brain, kidneys, and liver in mammals. In the brain, GS is found primarily in astrocytes, where it participates in the the detoxification of ammonia via assimilation and termination of neurotransmitter signals via recyclization. c-Myc promotes glutamine usage by upregulating glutaminase (GLS), which converts glutamine to glutamate in the TCA cycle, while in some cancer cells Myc-mediated thymine DNA glycosylase (TDG) transcription leads to GS upregulation due to TDG-dependent demethylation of the GS promoter. GS upregulation promotes cancer cell survival under glutamine limitation, while GS silencing decreases cancer cell proliferation and xenograft tumor growth. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Glutamine Synthetase, clone 11A3.1, Cat. No. MABN1182, is a highly specific mouse monoclonal antibody that targets Glutamine Synthetase, validated for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:20,000 dilution of from a representative lot detected Glutamine synthetase in 10 µg of human brain tissue lysate. Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Glutamine synthetase in human cerebellum, cerebral cortex, and tonsil tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant rat glutamine synthetase internal fragment. |
| Clone | 11A3.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 11A3.1 targets an internal epitope within the C-terminal half of human and rat glutamine synthetase. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~42 kDa observed. 42.06 kDa (human) and 42.27 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Glutamine synthetase in 10 µg of rat brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |