Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1191 |
| Description | Anti-Parvalbumin Antibody, clone 4E9.2 |
| Alternate Names |
|
| Background Information | Parvalbumin alpha (UniProt P02625; also known as PV) is encoded by the Pvalb (also known as Pva) gene (Gene ID 25269) in rat species. Parvalbumin alpha is a small (a.a. 2-110; Met1 removed posttranslationally) calcium-binding protein. Parvalbumin alpha is composed almost entirely of two EF-hand domains (a.a. 39-74 & 78-110) and is structurally related to calmodulin and troponin C. Parvalbumin (PV) is present in GABAergic interneurons in the nervous system, especially the reticular thalamus. PV interneurons are a subset of inhibitory GABAergic neurons that control the excitability of post-synaptic pyramidal neurons. Dysfunction or loss of PV interneurons is implicated in cognitive deficits associated with a number of well known psychiatric disorders, including schizophrenia, Alzheimer’s disease, and depression. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Parvalbumin Antibody, clone 4E9.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Parvalbumin. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Parvalbumin in human kidney, rat kidney, and mouse cerebral cortex tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of rat parvalbumin. |
| Epitope | Internal (N-terminal half). |
| Clone | 4E9.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4E9.2 detects an epitope conserved among human, mouse, and rat species. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g purified. |
| UniProt Number |
|
| Molecular Weight | ~12 kDa observed. 11.93 kDa (human), 11.80 kDa (mouse), 11.79 kDa (rat) calculated (Met1 removed). Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 0.25 µg/mL of this antibody detected Parvalbumin in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |