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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1530 |
| Description | Anti-Septin-3 Antibody, clone 5C1.2 |
| Alternate Names |
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| Background Information | Neuronal-specific septin-3 (UniProt Q9UH03) is encoded by the SEPT3 (also known as SEP3) gene (Gene ID 55964) in human. Septins are GTPases capable of forming higher-order oligomers and filamentous polymers that contact with cell membrane and cytoskeleton. Septins are involved in various cellular processes, including cytokinesis, karyokinesis, exocytosis, and cell shape maintenance. Septins exist in complexes that are composed of ordered non-polar arrays of multiple different septins. Human genome contains 13 septin genes that are grouped into the SEPT2 subgroup (septins 1, 2, 4, and 5), the SEPT3 subgroup (septins 3, 9, and 12), the SEPT6 subgroup (septins 6, 8, 10, 11, and 14), and the SEPT7 subgroup (septin 7). All subgroups contain the well conserved GTP-binding domain and differ mainly in their C-terminal region, where coiled-coil sequences are found in SEPT2, SEPT6, and SEPT7, but not SEPT3, subgroups. Septin-3 is the only septin to be expressed almost exclusively in brain tissue, where it is enriched in nerve terminals, suggesting a role in synaptic vesicle function. Increased septin-3 expression is reported in several brain tumours and SEPT3 polymorphisms are linked to Alzheimers disease. PKG-1 is shown to phosphorylate septin-3 in vitro, non-phosphorylated septin-3 localizes to the peripheral membrane, whereas the phosphorylated form is found in the cytosol. Murine and rat septin-3 is reported to form complexes with septin-5 and septin-7. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mm NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Septin-3, clone 5C1.2, Cat. No. MABN1153, is a highly specific mouse monoclonal antibody that targets Septin-3, validated for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:1,000 dilution of from a representative lot detected Septin-3 in 10 µg of human brain tissue lysate. Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Septin-3 in human cerebellum and cerebral cortex, as well as in mouse cerebral cortex tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugatged linear peptide coresponding to an internal sequence from the N-terminal half of human septin-3. |
| Clone | 5C1.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 5C1.2 targets an internal epitope present in human spliced isoforms 1&2 (SEP3A and SEP3B), both mouse and all three rat spliced isoforms (g-septin-alpha, -beta, and -gamma) reporeted by UniProt (Q9UH03, Q9Z1S5, and Q9WU34, respectively). Target epitope is absent from human spliced isoform 3 (SEP3C). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rat based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified. |
| UniProt Number |
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| Molecular Weight | ~40/39 kDa observed. 40.70/40.10 kDa (human isoform 1/2), 40.04/38.71 kDa (mouse isoform 1/2), and 40.60/39.62/34.30 kDa (rat isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Septin-3 in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |