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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1602 |
| Description | Anti-PIT-1 (POU1F1) Antibody, clone 8B6.1 |
| Alternate Names |
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| Background Information | Pituitary-specific positive transcription factor 1 (UniProt P28069; also known as GHF-1, Growth hormone factor 1, PIT-1) is encoded by the POU1F1 (also known as CPHD1, GHF1, PIT1) gene (Gene ID 5449) in human. PIT-1 is a transcription factor that serves as a master regulator of lineage commitment and expansion during the anterior pituitary development process that drives regional expansion and functional differentiation of five distinct hormone-producing cell lineages, including somatotropes, lactotropes, thyrotropes, corticotropes, and gonadotropes. Loss of Pit-1 expression results in a combined pituitary hormone deficiency (CPHD) syndrome reflecting the absence of lactotropes, somatotropes, and a subset of thyrotropes. Although originally characterized as pituitary-specific, PIT-1 is also expressed in human breast. PIT-1 upregulation in breast tumors increases cell proliferation and the expression of two breast cancer-related hormones, growth hormone (GH) and prolactin (PRL), leading to MMPs upregulation and breast cancer metastasis. Human POU1F1 gene encodes two alternatively spliced isoforms, where Pit-1A (GHF-2) a.a. 47-73 is truncated to a single Thr47 residue in Pit-1B (GHF-1). Both PIT-1 isoforms contain a PIT-1 POU-specific domain (a.a. 150-224 of Pit-1A or a.a. 124-198 of Pit-1B) and a DNA-binding Homeobox (a.a. 240-299 of Pit-1A or a.a. 214-273 of Pit-1B). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PIT-1 (POU1F1), clone 8B6.1 Antibody, Cat. No. MABN1602, is a highly specific mouse monoclonal antibody, that targets POU1F1 and has been tested in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human PIT-1 internal fragment. |
| Clone | 8B6.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 8B6.1 epitope lies within an internal region present in both human PIT-1 spliced isoform A (Pit-1A, GHF-2) and isoform B (Pit-1B, GHF-1). |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~33 kDa observed. 35.67 kDa (isoform A, Pit-1A, GHF-2) and 32.91 kDa (isoform B, Pit-1B, GHF-1) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human pituitary tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PIT-1/POU1F1 in 10 µg of human pituitary tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |