Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, ICC, IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1785-25UL |
| Description | Anti-CD166 (ALCAM) Antibody, clone TAG-1A3 |
| Alternate Names |
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| Background Information | CD166 antigen (UniProt: Q13740; also known as Activated leukocyte cell adhesion molecule, CD166) is encoded by the ALCAM (also known as MEMD) gene (Gene ID: 214) in human. CD166 is a cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6 and homotypic cell-cell contacts. It also promotes T-cell activation and proliferation via its interactions with CD6 and is shown to be involved in the formation and maturation of the immunological synapse via its interactions with CD6. CD166 mediates attachment of dendritic cells onto endothelial cells via homotypic interaction and inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. It has an extracellular (aa 28-527), transmembrane (aa 528-549), and a cytoplasmic (aa 550-583) domain. CD166 is expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. It is also detected on hematopoietic stem cells derived from umbilical cord blood, on lymph vessel endothelial cells, skin and tonsil, and on peripheral blood monocytes. Lower levels of CD166 are detected in spleen, placenta, and liver. CD166 is also detected in highly metastasizing melanoma cell lines. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-CD166 (ALCAM) Antibody, clone TAG-1A3, Cat. No. MABN1785, is a highly specific mouse monoclonal antibody that targets CD166 and has been tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected CD166 (ALCAM) in Flow Cytometry applications (Ding, Y., et. Al. (2014). MAbs. 6(6):1439-52). Immunocytochemistry Analysis: A representative lot detected CD166 (ALCAM) in Immunocytochmistry applications (Ding, Y., et. Al. (2014). MAbs. 6(6):1439-52). Immunoprecipitation Analysis: A representative lot detected CD166 (ALCAM) in Immunoprecipitation applications (Ding, Y., et. Al. (2014). MAbs. 6(6):1439-52). Flow Cytometry Analysis: A represetative lot detected CD166 (ALCAM) in human embryonic stem cells, human corneal stromal fibroblast (hCSF) cells, immortalized human corneal endothelial cells (B4G12), and human endothelial cells from donors (2) (Courtesy of Dr. Vanessa Ding, pH.D. and Dr. Andre Choo, pH.D, BTI, Singapore). Immunofluorescence Analysis: A representative lot detected CD166 (ALCAM) in Immunofluorescence applications (Ding, Y., et. Al. (2014). MAbs. 6(6):1439-52). Western Blotting Analysis: A representative lot detected CD166 (ALCAM) in Western Blotting applications (Ding, Y., et. Al. (2014). MAbs. 6(6):1439-52). |
| Biological Information | |
|---|---|
| Immunogen | Corneal endothelial cells (hCENC) isolated from donated human cadaves and immunized with monophosphoryl-lipid A trehalose dicorynomycolate (MPLCTDM) adjuvant. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone TAG-1A3 specifically detects CD166 in human. |
| Isotype | IgG1 |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~80 kDa observed; 65.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be human IgG1κ. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |