Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IF, ICC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1797-25UG |
| Description | Anti-Prdx-6 Antibody, clone TAG-2A12 |
| Alternate Names |
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| Background Information | Peroxiredoxin-6 (UniProt: P30041; also known as EC: 1.11.1.15, 1-Cys peroxiredoxin, 1-Cys PRX, 24 kDa protein, Acidic calcium-independent phospholipase A2, aiPLA2, Antioxidant protein 2, Liver 2D page spot 40, Non-selenium glutathione peroxidase, NSGPx) is encoded by the PRDX6 (also known as AOP2, KIAA0106) gene (Gene ID: 9588) in human. Peroxiredoxin-6 is a cytoplasmic protein that is involved in redox regulation of the cell. It reduces hydrogen peroxide and short chain organic fatty acid, and phospholipid hydroperoxides. It is the only mammalian 1-Cys member of the Prdx family with bifunctional - glutathione peroxidase and phospholipase A2 activities. Peroxiredoxin-6 shares several structural and functional properties with other members of the Prdx family, however, it has only one conserved cysteine residue and its thioredoxin does not participate in the catalytic cycle. Peroxiredoxin-6 is expressed in higher levels in epithelium such as apical regions of respiratory epithelium and skin epidermis and within the lung, it is expressed at relatively high levels within the type II alveolar epithelial cells and bronchiolar Clara cells. Lower levels of expression are reported in alveolar macrophages and microvascular endothelium. Human peroxiredoxin-6 shares 87% sequence identity with mouse, rat, and bovine. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Prdx-6 Antibody, clone TAG-2A12, Cat. No. MABN1797, is a highly specific mouse monoclonal antibody that targets Prdx-6 and has been tested in Flow Cytometry, Immunofluorescence, Immunocytochemistry and Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot detected Prdx-6 in Immunoprecipitation applications (Ding, V., et Al. (2014). MAbs. 6(6):1439-52). Immunocytochemistry Analysis: A representative lot detected Prdx-6 in Immunocytochemistry applications (Ding, V., et Al. (2014). MAbs. 6(6):1439-52). Flow Cytometry Analysis: A representative lot detected Prdx-6 in Flow Cytometry applications (Ding, V., et Al. (2014). MAbs. 6(6):1439-52). Immunofluorescence Analysis: A representative lot detected Prdx-6 in Immunofluorescence applications (Ding, V., et Al. (2014). MAbs. 6(6):1439-52). Western Blotting Analysis: A representative lot detected Prdx-6 in Western Blotting applications (Ding, V., et Al. (2014). MAbs. 6(6):1439-52). Flow Cytometry Analysis: A representative lot detected Prdx-6 in human corneal endothelial cells from a donor. (Courtesy of Dr. Vanessa Ding, pH.D. and Dr. Andre Choo, pH.D, BTI, Singapore). |
| Biological Information | |
|---|---|
| Immunogen | Corneal endothelial cells (hCENC) isolated from corneas of human cadavers and immunized with monophosphoryl-lipid A trehalose dicorynomycolate (MPLCTDM) adjuvant. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone TAG-2A12 detects Peroxiredoxin-6 in human cells. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~28 kDa observed; 25.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be human IgG1κ. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |