Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Chp | ELISA, ICC, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1801-25UG |
| Description | Anti-PMCA4b Antibody, clone JA3 |
| Alternate Names |
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| Background Information | Plasma membrane calcium-transporting ATPase 4 (UniProt: P23634; also known as EC: 3.6.3.8, PMCA4, Matrix-remodeling-associated protein 1, Plasma membrane calcium ATPase isoform 4, Plasma membrane calcium pump isoform 4) is encoded by the ATP2B4 (also known as ATP2B2, MXRA1) gene (Gene ID: 493) in human. PMCA4 is a multi-pass membrane protein that acts as a calcium/calmodulin-regulated and magnesium-dependent enzyme and catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. PMCA4 contains 2 calmodulin binding domains known as Domain A (aa 1086-1103) and B (aa 1104-1113). PMCA4 is shown to be a regulator of neuronal nitric-oxide synthase (nNOS) and is suggested to play a structural role in tethering nNOS to a highly compartmentalized domain in the cardiac cell membrane. PMCA4b is also a major PMCA expressed in developing mammary tissue. PCMA4b form contains a PDZ domain that lies within the last 7 amino acids of the carboxyl terminus in mouse, which is absent in PCMAa tail. The kinetics of binding of Ca2+/CaM to PMCA4b is very slow, but remains active for longer periods when compared to PMCA4a. A significantly reduced migration of PCMA4b expressing in B-RAF mutant cells is linked to their reduced metastatic potential. (Ref.: Afronze, T., et al. (2014). J. Biol. Chem.289 (10), 7221-31); Mohanmed TM et al. (2011). J. Biol. Chem. 286(48):41520-29). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-PMCA4b, clone JA3, Cat. No. MABN1801, is a highly specific mouse monoclonal antibody that targets PMCA4b and has been tested in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected PMCA4b in Western Blotting applications (Caride, A.J., et. al. (1996). Biochem J. 316 (Pt 1):353-9; Borke, J.L., et. al. (1987). J clin Invest. 80(5):1225-31; DeMarco, S.J., et. al. (2002). J Biol Chem. 277(12):10506-11; Gros, R., et. al. (2003). Circ Res. 93(7):614-21; Paszty, K., et. al. (2015). Sci Signal. 8(364):ra19; Usachev. M., et. al. (2001). J Neurochem. 76(6):1756-65). Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected PMCA4b in human cerebral cortex, human kidney, and bovine cornea tissues. Immunohistochemistry Analysis: A representative lot detected PMCA4b in Immunohistochemistry applications (Borke, J.L., et. al. (1987). J clin Invest. 80(5):1225-31). Immunocytochemistry Analysis: A representative lot detected PMCA4b in Immunocytochemistry applications (Usachev. M., et. al. (2002). J Neuron. 33(1):113-22). ELISA Analysis: A representative lot detected PMCA4b in ELISA applications (Caride, A.J., et. al. (1996). Biochem J. 316 (Pt 1):353-9; Borke, J.L., et. al. (1987). J clin Invest. 80(5):1225-31). |
| Biological Information | |
|---|---|
| Immunogen | Purified plasma membrane calcium-transporting ATPase from human red blood cells. |
| Clone | JA3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone JA3 detects Plasma membrane calcium-transporting ATPase in human brain tissue. It targets an epitope with in 25 amino acids from the C-terminal region. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Chimpanzee. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~134 kDa observed; 137.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human brain tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected PMCA4b in 10 µg of human brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |