| Description | |
|---|---|
| Catalogue Number | MABN1840-100UG |
| Description | Anti-Neuritin (CPG15) Antibody, clone A6.1 |
| Alternate Names |
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| Background Information | Neuritin (UniProt: Q9NPD7) is encoded by the NRN1 (also known as NRN) gene (Gene ID: 51299) in human. Neuritin, a conserved, glycosylphosphatidylinositol (GPI)-anchored protein that plays a role in the development, survival and function of neurons. Its activity is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3). It is highly expressed by induced and natural regulatory T cells (TREGs). It also promotes the maintenance of the TREGs pool by stabilizing Foxp3 expression and promoting the persistence of these cells. Deficiency of Neuritin is shown to result in an imbalance between central and peripheral TREG populations and functions. Neuritin has a wide variety of effects in the nervous system, such as promoting neurite outgrowth, modulating neurite outgrowth during neuronal differentiation, and protecting motor neuron axons. It can also promote dendritic growth, regulate synaptic plasticity, and stabilize active synapses, and promote synaptic maturation. Its expression in the hippocampus is shown to be induced by neuronal activity following chemical- or electrical-induced seizures, ischemia, and exercise. Chronic antidepressant treatment is reported to increase neuritin expression in rat brain. Neuritin is synthesized with a signal peptide (aa 1-27) and a propeptide (aa 28-116) that are cleaved off in the mature form (aa 117-142). (Ref.: Son, H., et Al. (2012). Proc. Natl. Acad. Sci. USA. 109(28); 11378-83; Zhou, S., and Zhou, J (2014). Curr. Med. Chem. 21(10); 1212-19). Note: A6.1 hybridoma clone, and WT/KO validation, courtesy of Lauren Makuch and Dr. Elly Nedivi, Massachusetts Institute of Technology. Note: A6.1 hybridoma clone, and WT/KO validation, courtesy of Dr. Lauren Makuch and Dr. Elly Nedivi, Massachusetts Institute of Technology. |
| Product Information | |
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| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-Neuritin (CPG15), clone A6.1, Cat. No. MABN1840, is a highly specific mouse monoclonal antibody that targets Neuritin and has been tested for use in Western Blotting. |
| Key Applications |
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| Biological Information | |
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| Immunogen | GST-tagged full-length human recombinant Neuritin. |
| Clone | A6.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone A6.1 detects human and rat Neuritin. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Rat. Predicted to react with Bovine and Monkey based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~15 kDa observed; 15.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting with recombinant human/rat neuritin. Western Blotting Analysis: 0.5 µg/mL of this antibody detected recombinant human/rat Neuritin (CPG15). |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 100 μg |