Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN2257 |
| Description | Anti-Nudel (NDEL1) Antibody, clone 15D5.1 |
| Alternate Names |
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| Background Information | Nuclear distribution protein nudE-like 1 (UniProt: Q9GZM8; also known as Protein Nudel, Mitosin-associated protein 1) is encoded by the NDEL1 (also known as EOPA, MITAP1, NUDEL) gene (Gene ID: 81565) in human. Nudel protein belongs to the nude family and is expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. Its expression is shown to peak during mitosis. Nudel is undergoes phosphorylation during mitosis. Three isoforms of Nudel have been reported that are produced by alternative splicing. Nudel is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. It may regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome and is shown to positively regulate the activity of the minus-end directed microtubule motor protein dynein. It enhances dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends and is shown to be essential for several dynein- and microtubule-dependent processes, such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Nudel is required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate and also plays a role in the regulation of neurite outgrowth. It localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Nudel (NDEL1), clone 15D5.1, Cat. No. MABN2257, is a highly specific mouse monoclonal antibody that targets Nuclear distribution protein nudE-like 1 (Nudel) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Nuclear distribution protein nudE-like 1 (Nudel) in human colon, human cerebral cortex, and human tonsil tissues. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-taged recombinant fragment corresponding to 101 amino acids from the N-terminal region of human Nuclear distribution protein nudE-like 1 (Nudel). |
| Clone | 15D5.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 15D5.1 detects Nuclear distribution protein nudE-like 1 (Nudel) in human and murine cells. It targets an epitope within 101 amino acids from the N-terminal region. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~40 kDa observed; 38.38 kda calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Nuclear distribution protein nudE-like 1 (Nudel) in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |