Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | DB, ELISA, IH(P), IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN2275-25UG |
| Description | Anti-phospho-Tau (Ser235) Antibody, clone RN235 |
| Alternate Names |
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| Background Information | Microtubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Tau, a highly asymmetric and heat-stable protein, is expressed mainly in the brain, where it regulates the stability and orientation of microtubules in neurons, astrocytes, and oligodendrocytes. Tau protein is reported to be the predominant component of the paired helical filaments (PHFs) and neurofibrillary tangles (NFTs) that are characteristic of pathological brain lesions in Alzheimer s disease. The dephosphorylation of Tau protein promotes rapid and extensive microtubule polymerization, while the phosphorylation of Tau decreases its ability to promote microtubule assembly. Tau is a substrate for a multitude of protein kinases, including CaM kinase II, casein kinase II, protein kinase A (PKA), MAP kinase (ERK2), Cdk5/ p35, and glycogen synthase kinase-3 (GSK-3) and is phosphorylated at many different sites in Alzheimer s disease brain. It is believed that the phosphorylation of specific sites, rather than the overall extent of phosphorylation, is important in modulating the ability of Tau to bind microtubules and promote microtubule assembly. This antibody (clone RN235) specifically reacts with tau phosphorylated at serine 235, and detects intraneuronal NFTs (iNFTs), and extraneuronal NFTs (eNFTs) in the brain tissue. Although theoretically it can also react with phospho Thr231, but EC50 is very high compared to reactivity with phospho Ser235 (407 nM vs 0.61 nM). (Ref.: Brici, D., et Al. (2018). J. Alzheimer s Dis. 61(3); 899-905). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-phospho-Tau (Ser235), clone RN235, Cat. No. MABN2275, is a highly specific mouse monoclonal antibody that targets Microtubule-associated protein tau and has been tested for use in DOT Blot, ELISA, Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected phospho-tau (Ser235) in human Alzheimers diseas (AD) brain tissue sections. DOT Blot analysis: A representative lot detected phosphorylated recombinant human tau (pSer235), but not the non-phosphorylated recombinant human tau (Tau). (Courtesy of Rebecca Nisbet, pH.D., University of Queensland, Brisbane, Austraila). Immunofluorescence Analysis: A representative lot detected phospho-tau (Ser235) in K3 and rTg4510 tau transgenic mouse brains (Courtesy of Rebecca Nisbet, pH.D., University of Queensland, Brisbane, Austraila). Immunohistochemistry (Paraffin) Analysis: A representative lot detected phospho-Tau (Ser235) in human Alzheimer s disease (AD) brain tissue sections. (Courtesy of Rebecca Nisbet, pH.D., University of Queensland, Brisbane, Austraila). ELISA Analysis: A representative lot full-length human recombinant phospho-tau (Ser235) or GSK-3 beta phosphorylated tau (pTau), but not the non-phosphorylated tau (Courtesy of Rebecca Nisbet, pH.D., University of Queensland, Brisbane, Austraila). Western Blotting Analysis: A representative lot detected phospho-tau (pSer235) K3 tau transgenic mouse brain homogenates, but not in homogenates from tau knock-out (KO) (Courtesy of Rebecca Nisbet, pH.D., University of Queensland, Brisbane, Austraila). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phospho serine 235 of human tau-C. |
| Clone | RN235 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone RN235 is a mouse monoclonal antibody that detects microtubule-associated protein tau (MAPT) phosphorylated on serine 235 of isoform Tau-C. It targets an epitope with in 12 amino acids surround pSer235. This sequence is also conserved in isoform Tau-F. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~55 kDa observed; 42.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in brain tissue lysates from either K3 transgenic mouse or MAPT KO mouse. Western Blotting Analysis: 2 µg/mL of this antibody detected Microtubule-associated protein tau (pSer235) in brain tissue lysates from K3 transgenic mouse, but not in brain of microtubule-associated protein tau (MAPT) knock out mouse. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |