Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, ICC, IP, WB, FC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN2297 |
| Description | Anti-APP Antibody, clone 7H6 |
| Alternate Names |
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| Background Information | Amyloid beta A4 protein (UniProt: P05067; also known as ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. APP can also be cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. In addition to its obvious role in Alzheimers disease, the most-substantiated role for APP is in synaptic formation and repair. Its expression is upregulated during neuronal differentiation and after neural injury. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-APP, clone 7H6, Cat. No. MABN2297, is a highly specific rat monoclonal antibody, that targets Amyloid beta A4 protein and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected APP in HEK293 cells. Western Blotting Analysis: A representative lot detected APP in Western Blotting applications (Hoefling, C., et. Al. (2016). Aging Cell. 15(5):953-63). Flow Cytometry Analysis: A representative lot detected APP in Flow Cytometry applications (Hoefling, C., et. Al. (2016). Aging Cell. 15(5):953-63). Western Blotting Analysis: A 1:10 dilution from a representative lot detected App in WT and APP knockdown HEK293T cells (Courtesy of Dr. med. Peer-Hendrik Kuhn, pH.D., Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Munich, Germany). Immunoprecipitation Analysis: A representative lot detected APP in Immunoprecipitation applications (Hoefling, C., et. Al. (2016). Aging Cell. 15(5):953-63). ELISA Analysis: A representative lot detected APP in ELISA applications (Hoefling, C., et. Al. (2016). Aging Cell. 15(5):953-63). Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected APP in HEK293T either lentivirally transduced with APP shRNA-1 or APP shRNA-2 both coexpressing GFP as a reporter (Courtesy of Dr. med. Peer-Hendrik Kuhn, pH.D., Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Munich, Germany). Immunocytochemistry Analysis: A representative lot detected APP in Immunocytochemistry applications (Hoefling, C., et. Al. (2016). Aging Cell. 15(5):953-63). |
| Biological Information | |
|---|---|
| Immunogen | StrepII-tagged human recombinant ectodomain region of the neuronal isoform hAPP695 lacking the KPI domain. |
| Clone | 7H6 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 7H6 specifically detects human amyloid precursor protein (APP) and does not display reactivity with other species. It targets an epitope within the ectodomain region of APP. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~95 kDa observed; 86.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected APP in 10 µg of HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |