| Description | |
|---|---|
| Catalogue Number | MABN2398-25UL |
| Description | Anti-Klotho Antibody, clone KL-115 |
| Alternate Names |
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| Background Information | Klotho (UniProt: Q9UEF7; also known as EC: 3.2.1.31) is encoded by the KL gene (Gene ID:9365) in human. Klotho is a homodimeric, highly N-glycosylated, single-pass type I membrane protein that is present in kidney, placenta, small intestine, and prostate tissue. In kidney it is predominantly present in cortical renal tubules. A soluble secreted form (isoform 2, ~ 64 kDa) is present in serum and cerebrospinal fluid. The Klotho peptide generated by cleavage of the membrane-bound isoform (Isoform 1) is considered as an anti-aging circulating hormone that is reported to extend life span by inhibiting insulin/IGF1 signaling. It also acts as a cofactor for interaction of FGF23 with FGF R1, which negatively regulates the activity of 1-alpha-hydroxylase, the rate limiting enzyme in the synthesis of 1,25(OH)2D3. Klotho is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to generate the mature form that has an extracellular domain (aa 31-981), a short transmembrane domain (aa 982-1002), and a short cytoplasmic tail (aa 1003-1012). Deficiency of Klotho is reported to shorten life-span in animal models with a myriad of disorders associated with old age, such as arteriosclerosis, osteoporosis, and skin atrophy. Klotho deficient mice also display severe hyperphosphatemia and soft tissue calcification. On the contrary, over-expression of Klotho is shown to extend life-span in murine models by about 30% with side effects, such as mild insulin resistance and enhanced resistance to oxidative stress. Defects in KL gene has been linked to chronic renal failure complications. |
| Product Information | |
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| Format | Unpurified |
| Presentation | Rat monoclonal antibody in ascites with 0.1% sodium azide with 50% glycerol. |
| Applications | |
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| Application | Anti-Klotho, clone KL-115, Cat. No. MABN2398, is a highly specific rat monoclonal antibody that targets klotho and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A represetative lot detected Klotho in Western Blotting applications (Maltare, A., et. Al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7). Immunocytochemistry Analysis: A represetative lot detected Klotho in Immunocytochemistry applications (Maltare, A., et. Al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7). Immunoprecipitation Analysis: A represetative lot immunoprecipitated Klotho in Immunoprecipitation applications (Maltare, A., et. Al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 207 amino acids from the extracellular domain from the N-terminal region of human Klotho. |
| Clone | KL-115 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone KL-115 is a rat monoclonal antibody that specifically detects human Klotho. It targets an epitope with in 207 amino acids from the KL1 domain from the N-terminal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~130 kDa observed; 116.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in human kidney tissue lysates. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Klotho in 10 µg of human kidney tissue lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
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| Material Size | 25 µL |