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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN24 |
| Description | Anti-pan-Shank Antibody, clone N23B/49 |
| Alternate Names |
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| Background Information | Shank1, Shank2, and Shank3 make up a family of proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank contains multiple domains for protein-protein interaction including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile α motif domain, and a proline-rich region. Alternative splicing in the Shank family may be a mechanism that regulates the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect pan-Shank using this Anti-pan-Shank Antibody, clone N23B/49 validated for use in WB, IH(P). |
| Key Applications |
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| Application Notes | Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Shank in rat cerebellum tissue. Western Blot Analysis: A previous lot of this antibody detected Shank in extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids. Courtesy of James Trimmer, UC Davis/NIH NeuroMab Facility. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein consisting of SH3/PDZ domain of rat Shank2. |
| Clone | N23B/49 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G |
| UniProt Number |
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| UniProt Summary | FUNCTION: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. SUBUNIT STRUCTURE: Interacts with DNM2 and BAIAP2. Interacts with GRID2 (By similarity). Interacts with CCTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with SLC9A3 and PLCB3. Interacts with DBNL. Interacts with CFTR. SUBCELLULAR LOCATION: Cytoplasm. Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell projection › growth cone. Note: Cytoplasm, postsynaptic density of neuronal cells. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. TISSUE SPECIFICTY: All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level). DEVELOPMENTAL STAGE: Expressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum. DOMAIN: The PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR. SEQUENCE SIMILARIITIES: Belongs to the SHANK family. Contains 1 PDZ (DHR) domain. Contains 1 SAM (sterile alpha motif) domain. Contains 1 SH3 domain. |
| Molecular Weight | ~160 kDa observed. Other isoforms may be observed in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in rat brain membrane tissue lysate. Western Blot Analysis: 2 µg/mL of this antibody detected Shank on 10 µg of rat brain membrane tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |