Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | EM, ICC, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN2416-25UG |
| Description | Anti-HSD17B10 Antibody, clone 5F3 |
| Alternate Names |
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| Background Information | 3-hydroxyacyl-CoA dehydrogenase type-2 (UniProt: Q99714; also known as 17-beta-hydroxysteroid dehydrogenase 10, 17-beta-HSD 10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 3-hydroxyacyl-CoA dehydrogenase type II, Endoplasmic reticulum-associated amyloid beta-peptide-binding protein, Mitochondrial ribonuclease P protein 2, Mitochondrial RNase P protein 2, Short chain dehydrogenase/ reductase family 5C member 1, Short-chain type dehydrogenase/reductase XH98G2, Type II HADH) is encoded by the HSD17B10 (also known as ERAB, HADH2, MRPP2, SCHAD, SDR5C1, XH98G2) gene (Gene ID: 3028) in human. 3-hydroxyacyl-CoA dehydrogenase type-2 is a part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5-ends. It functions in mitochondrial tRNA maturation. It catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. It catalyzes the third step in the beta-oxidation of fatty acids and also carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. With C21 steroids it exhibits 20-beta-OH and 21-OH dehydrogenase activities. May contribute to neuronal dysfunction associated with Alzheimer disease (AD) via interactions with intracellular amyloid-beta peptides. 3-hydroxyacyl-CoA dehydrogenase type-2 is ubiquitously expressed in normal tissues, but is overexpressed in neurons affected in AD. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-HSD17B10 antibody, clone 5F3, Cat. No. MABN2416, is a highly specific mouse monoclonal antibody that targets HSD17B10 and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Electron Microscopy Analysis: A representative lot detected HSD17B10 in Electron Microscopy applications (Wen, g.Y., et. Al. (2002). Brain Res. 954(1):115-22 ). Immunocytochemistry Analysis: A representative lot detected HSD17B10 in Immunocytochemistry applications (Frackowiak, J., et. Al. (2001). Brain Res. 907(1-2):44-53 ). Western Blotting Analysis: A representative lot detected HSD17B10 in Western Blotting applications (Frackowiak, J., et. Al. (2001). Brain Res. 907(1-2):44-53 ). Immunohistochemistry Analysis: A 1:250-1:1,000 dilution from a representative lot detected HSD17B10 in human placenta and human breast cancer tissues. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged full length recombinant human 3-hydroxyacyl-CoA dehydrogenase type-2 protein (ERAB/HADH). |
| Clone | 5F3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 5F3 detects 3-hydroxyacyl-CoA dehydrogenase type-2 in human and murine cells. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293T cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected HSD17B10 in 10 µg of HEK293T cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |