Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN2419-25UG |
| Description | Anti-phospho-alpha-synuclein (Ser129) Antibody, clone LS4-1B1 |
| Alternate Names |
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| Background Information | Alpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is a membrane-bound protein in dopaminergic neurons that is involved in the regulation of dopamine release and transport. It is also reported to induce fibrillization of microtubule-associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli. Aggregation of alpha-synuclein is known to be a causative factor in the development of Parkinsons disease (PD), which leads to the degeneration of dopaminergic neurons in the substantia nigra (SN) in the midbrain region. Aggregates of alpha-synuclein are present in Lewy bodies. Alpha-synuclein can be phosphorylated, predominantly on serine residues by casein kinase 1. Phosphorylation of Ser129 is reported to be selective and extensive in synucleinopathy lesions and promotes insoluble fibril formation. Mutations in the SNCA gene produce defective alpha-synuclein that induces conformational changes making it more prone to self-aggregation and deposition in Lewy bodies. Mutant forms of alpha-synuclein impair synaptic vesicle formation, elevate cytoplasmic levels of dopamine, and increases superoxide radicals, which lead to oxidative stress and misfolding of alpha-synuclein. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho-alpha-synuclein (Ser129), clone LS4-1B1, Cat. No. MABN2419, is a highly specific mouse monoclonal antibody that targets alpha-synuclein and neurofilament light polypeptide (NFL) phosphorylated on Ser129 and has been tested in Western Blotting and Immunohistochemistry.. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected phospho-alpha-synuclein (Ser129) in Western Blotting applications (Rutherford, N.J., et. Al. (2016). Acta Neuropathol Commun. 4(1):80). Immunohistochemistry Analysis: A representative lot detected phospho-alpha-synuclein (Ser129) in Immunohistochemistry applications (Rutherford, N.J., et. Al. (2016). Acta Neuropathol Commun. 4(1):80). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptides corresponding to aa 121-137 and 124-134 phosphorylated on Serine 129 from the C-terminal region of human alpha synuclein. |
| Clone | LS4-1B1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone LS4-1B1 specifically detects alpha-synuclein phosphorylated on serine 129. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~70 kDa observed (aggregated); 14.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected phospho-alpha-synuclein (Ser129) in mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |