Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, B, M | IHC, IP, IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN2439-25UG |
| Description | Anti-ABCA4 Antibody, clone 3F4 |
| Alternate Names |
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| Background Information | Retinal-specific ATP-binding cassette transporter (UniProt F1MWM0/P78363; also known as ATP-binding cassette sub-family A member 4, ABC transporter A4, RIM ABC transporter, RIM Protein) is encoded by the ABCA4 gene in bovine species. ABC transporter A4, a ~250 kDa single-chain, is a multi-pass membrane protein of the superfamily of ATP-binding cassette (ABC) transporters that is localized to the disk margins of vertebrate photoreceptor outer segments. It is implicated in the clearance of all-trans retinal derivatives from rod and cone photoreceptor cells following photoexcitation as part of the visual cycle. ABC transporter A4 is composed of two symmetrically organized halves, each comprising six membrane-spanning helices, a large glycosylated exocytoplasmic domain located inside the disk, and a cytoplasmic domain with an ATP-binding cassette. Mutations in ABCA4 are known to cause Stargardt macular degeneration, which is an autosomal recessive form of juvenile macular degeneration associated with a severe loss in vision. (Ref.: Tsybovsky, Y et Al. (2010). Adv. Exp. Med. Biol. 703, 105-125). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-ABCA4, clone 3F4, Cat. No. MABN2439, is a highly specific mouse monoclonal antibody that targets ABCA4 (RIM) and has been tested in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ABCA4 in mouse retina cells. Immunofluorescence Analysis: A 1:250 dilution from a representative lot detected ABCA4 in mouse retina cells (Courtesy of Dr. Robert Molday, pH.D., University of British Columbia, Canada). Electron Microscopy Analysis: A representative lot detected ABCA4 in Electron Microscopy applications (Illing, M., et. Al. (1997). J Biol Chem. 272(15):10303-10). Immunoprecipitation Analysis: A representative lot detected ABCA4 in Immunoprecipitation applications (Bungert, S., et. Al. (2001). J Biol Chem. 276(26):23539-46). Western Blotting Analysis: A representative lot detected ABCA4 in Western Blotting applications (Molday, L.L., et. Al. (2000). Nat Genet. 25(3):257-8; Illing, M., et. Al. (1997). J Biol Chem. 272(15):10303-10; Beharry, S., et. Al. (2004). J Biol Chem. 279(52):53972-9). Immunohistochemistry Analysis: A representative lot detected ABCA4 in Immunohistochemistry applications (Molday, L.L., et. Al. (2000). Nat Genet. 25(3):257-8). |
| Biological Information | |
|---|---|
| Immunogen | Whole purified bovine disk rim protein. |
| Clone | 3F4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3F4 detects ABC transporter (ABCA4) in Bovine and Human cells. It targets an epitope within 9 amino acids from the C-terminal end. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human, Bovine, Mouse |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~220 kDa observed; 257.38 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human retina tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected ABCA4 in 10 µg of H uman retinal tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |