Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | ICC, IH(P), IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN2443-25UL |
| Description | Anti-nonphospho-GSK3 (Ser9) Antibody, beta specific Antibody, clone 12B2 |
| Alternate Names |
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| Background Information | Glycogen synthase kinase-3 beta (UniProt: P49841; also known as EC: 2.7.11.26, GSK-3 beta, Serine/threonine-protein kinase GSK3B) is encoded by the GSK3B gene (Gene ID: 2932) in human. GSK-3 is a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways during embryogenesis and in metabolic control. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3 alpha and a 47 kDa GSK-3 beta. The GSK-3 alpha contains a glycine-rich extension at its N-terminal. GSK-3 is constitutively active in cells and is regulated through inhibition of its activity. GSK-3 shows a preference for target proteins that are pre-phosphorylated at a `priming residue located C-terminal to the site of GSK-3 phosphorylation. Priming phosphorylation, although not absolutely required, enhances the efficiency of phosphorylation of most GSK-3 substrates. Phosphorylation of a threonine residue in the activation loop (T-loop) is considered to be essential for its activity. The T-loop of GSK-3 beta is phosphorylated at Tyr216, which play a role in forcing open the substrate-binding site of the enzyme. A primary mechanism of GSK-3 beta activity regulation is phosphorylation of N-terminal serine residues (Serine 9). Phosphorylation at this site is inhibitory to GSK-3 beta kinase activity because the phosphorylated N-terminus acts as a competitive inhibitor for primed substrates. GSK-3 beta is constitutively active in resting cells and treatment of cells with an agent, such as insulin, causes GSK-3 inactivation through a PI 3-kinase (PI 3-K)-dependent mechanism. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without preservatives. |
| Applications | |
|---|---|
| Application | Anti-nonphospho-GSK3 (Ser9), beta specific, clone 12B2, Cat. No. MABN2443, is a highly specific mouse monoclonal antibody that targets Glycogen synthase kinase-3 beta and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected nonphospho-GSK3 (Ser9) in human and mouse brain tissue lysates. ELISA Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in ELISA applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123). Western Blotting Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123). Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected nonphospho-GSK3 (Ser9) in human cerebral cortex tissue. Immunocytochemistry Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123). Immunoprecipitation Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123). Immunohistochemistry Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated three linear peptides from the N-terminal region of human GSK-3 beta. |
| Clone | 12B2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 12B2 detects GSK-3 beta in human, murine, and rat cells. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~45 kDa observed. 46.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected nonphospho-GSK3 (Ser9) in rat brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |