Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H | FC, IHC, IF, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN2471-25UG |
| Description | Anti-Aquaporin-4 Antibody, clone mECD |
| Alternate Names |
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| Background Information | Aquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase g in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NMO) from multiple sclerosis. Clone mECD is shown to induce clustering and internalization of Aquporin-4 and cause its redistribution, which is also observed with NMO-IgG. Exposure to mECD antibody is also shown to cause coaggregation of astrocytic CD28B and Aquaporin-4. (Ref.: Hinson, SR et Al. (2017). Proc. Natl.Acad. Sci. USA 114(21); 5491-5496). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without preservatives. |
| Applications | |
|---|---|
| Application | Anti-Aquaporin-4, clone mECD, Cat. No. MABN2471, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected Aquaporin-4 in Flow Cytometry applications (Hinson, S.R., et. Al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496). Immunofluorescence Analysis: A representative lot detected Aquaporin-4 in Immunofluorescence applications (Hinson, S.R., et. Al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496). Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprcipitation applications (Hinson, S.R., et. Al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full-length human recombinant Aquaporin-4. |
| Epitope | extracellular domain |
| Clone | mECD |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone mECD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in the extracellular region. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 34.83 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in rat brain frozen tissue sections. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Aquaporin-4 in Rat brain frozen tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |