| Description | |
|---|---|
| Catalogue Number | MABN2475-25UG |
| Description | Anti-NMDAR2B Antibody, clone 11D1 |
| Alternate Names |
|
| Background Information | Glutamate receptor ionotropic, NMDA2B (UniProt: Q13224; also known GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, N-methyl-D-aspartate receptor subunit 3, NR3, hNR3) is encoded by the GRIN2B (also known as NMDAR2B) gene (Gene ID: 2904) in human. NMDA receptor complexes are ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. They are involved in long-term potentiation and neuronal plasticity and regulate the number of nerve cells during development. However, over-stimulation NMDA receptors can lead to neuronal degeneration. NMDAR2B is a heterotetrameric, multi-pass membrane protein that is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off in the mature form. The heterotetrameric channel is composed of two zeta subunits and two epsilon subunits, but can also form channels that contain at least one zeta subunit and at least one epsilon subunit. Activation of NMDA 2B channel requires binding of glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by magnesium ions. NMDA 2B is reported to be phosphorylated at Ser1303 by DAPK1 that enhances its channel activity allowing injurious Ca2+ influx that results in neuronal death. Mutations in NMDAR2B have been linked to early infantile epileptic encephalopathy that is characterized by refractory seizures and neurodevelopmental impairment. Some mutations are also known to cause mental retardation with or without seizures. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-NMDAR2B, clone 11D1, Cat. No. MABN2475, is a highly specific mouse monoclonal antibody that targets Glutamate receptor ionotropic, NMDA 2B and has been tested for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected NMDAR2B in Western Blotting applications (Lu, W., et. Al. (2017). Science. 355(6331)). Western Blotting Analysis: 4 µg/mL from a representative lot detected NMDAR2B in mouse brain tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | Full length purified human Glutamate receptor ionotropic, NMDA 2B (NMDAR2B). |
| Clone | 11D1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 11D1 is a mouse monoclonal antibody that specifically detects NMDAR2B. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
|
| Molecular Weight | 166 kDa observed; 166.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: 4 µg/mL of this antibody detected NMDAR2B in rat brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |