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Millipore This Anti-Synaptotagmin-12 Antibody (Syt12), Clone 1b12.1 Is Validated For Use In Western Blotting For The Detection Of Synaptotagmin-12 (Syt12). - Mill (Additional S&H or Hazmat Fees May Apply)
Synaptotagmin-12 (SYT12) , also known as Synaptotagmin-12, Synaptotagmin XII, SytXII, and encoded by the gene SYT12, is an important protein involved in calcium dependent exocytosis of secretory vesicles through calcium and phospholipid binding to the C2 domain and Synaptotagmin-12 may serve as calcium sensors in the process of vesicular trafficking and exocytosis. Synaptotagmin-12 is a synaptic vesicle phosophoprotein that modulates spontaneous neurotransmitter release, but unlike other family members Synaptotagmin-12 differs from classical synaptotagmins in that it does not bind Ca(2+). In synaptic vesicles, synaptotagmin-12 forms a complex with synaptotagmin-1 that prevents synaptotagmin-1 from interacting with SNARE complexes. Synaptotagmin-12 also is an abundant synaptic vesicle protein that is important in LTP transmission. For instance, its phosphorylation by cAMP Protein Kinase is necessary for hippocampal mossy fiber LTP.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Synaptotagmin-12 Antibody (SYT12), clone 1B12.1 is validated for use in Western Blotting for the detection of Synaptotagmin-12 (SYT12).
Key Applications
Western Blotting
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the cytoplasmic domain of human Synaptotagmin-12 (SYT12).
Epitope
Cytoplasmic domain
Clone
1B12.1
Concentration
0.5 mg/mL
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Rat
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001171351
Gene Symbol
SYT12
Purification Method
Protein g Purified
UniProt Number
Q8IV01
Molecular Weight
~ 47 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptotagmin-12 (SYT12) in 200 µg of rat brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
