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Millipore This Anti-Chrdl1 Antibody, Clone 10c9.3 Is Validated For Use In Western Blotting And Immunohistochemistry For The Detection Of Chrdl1. - Mill (Additional S&H or Hazmat Fees May Apply)
Chordin-like protein 1 (CHRDL1) is an important secreted developmental mediator protein expressed in developing cornea, retina, brain, cerebellum and neocortex and developing bone. CHRDL1 in the nervous system alters the fate commitment of neural stem cells from gliogenesis to neurogenesis and contributes to neuronal differentiation of neural stem cells in the brain by the preventing the adoption of a glial fate. CHRDL1 also plays a critical role in dorsventral axis formation and regulates the action of BMP4 and preventing it from interacting with its receptors. In the developing eye CHRDL1 regulates retinal angiogenesis and the development of the anterior segment of the eye. Mutations in CHRDL1 are associated with various eye disorders and in particular one named Megalocornea 1, an X-linked developmental disease.
Product Information
Format
Purified
Control
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected CHRDL1 in 200 µg of human brain tissue lysate. Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected CHRDL1 in human retina, human cerebellum, rat cerebellum, and rat cerebral cortex tissue.
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-CHRDL1 Antibody, clone 10C9.3 is validated for use in Western Blotting and Immunohistochemistry for the detection of CHRDL1.
Key Applications
Western Blotting
Immunohistochemistry
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human CHRDL1.
Clone
10C9.3
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Rat
Species Reactivity Note
Human, Rat.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001137453
Gene Symbol
CHRDL1
NRLN1
Purification Method
Protein G Purified
UniProt Number
Q9BU40
Molecular Weight
~65 kDa observed. The calculated molecular weight of this protein is 50 kDa, but can be observed at ~65 kDa due to high glycosylation.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected CHRDL1 in 200 µg of rat brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
