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Millipore Detect Vdac Using This Mouse Monoclonal Antibody, Anti-Vdac1 Antibody, Clone N152b/23 Validated For Use In Western Blotting & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
Voltage-dependent anion-selective channel protein 1
VDAC-1
hVDAC1
Outer mitochondrial membrane protein porin 1
Plasmalemmal porin
Porin 31HL
Porin 31HM
Background Information
Voltage-dependent anion-selective channel protein 1 (VDAC1, VDAC), also known as outer mitochondrial membrane protein porin 1, is the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC1 forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. VDAC1 may participate in the formation of the permeability transition pore complex (PTPC), which is responsible for the release of mitochondrial products that triggers apoptosis.
Product Information
Format
Purified
Control
Mouse brain tissue lysate
Presentation
Purified mouse monoclonal IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect VDAC using this mouse monoclonal antibody, Anti-VDAC1 Antibody, clone N152B/23 validated for use in western blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blotting Analysis: A representative lot states VDAC1 does not cross-react with VDAC2 or VDAC3 (based on KO validation results) representative lot deteted VDAC1 in wild type mouse liver tissue, and demonstrated a loss of signal in VDAC1 knock out mouse hippocampal tissue using immunobloting analysis (Prof. J. Trimmer, University of California, Davis). Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected VDAC1 in human cardiac myocytes tissue.
Biological Information
Immunogen
Recombinant protein corresponding to human VDAC1.
Clone
N152B/23
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
This antibody does not cross-react with VDAC2 or VDAC3 (Prof. J. Trimmer, University of California, Davis).
Isotype
IgG2aκ
Species Reactivity
Human
Mouse
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_003365
Gene Symbol
VDAC1
VDAC
Purification Method
Protein g Purified
UniProt Number
P21796
Molecular Weight
~ 35 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected VDAC1 in 10 µg of mouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
