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Millipore Detect Cox-1 Using This Anti-Cox-1 Antibody, Clone 5c5.1 Validated For Use In Western Blotting & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
Cox-1, also known as Cytochrome C oxidase subunit 1, Cytochrome C oxidase polypeptide I, and encoded by the gene MT-CO1, CO1, COX1, MTCO1, is the catalytic subunit of Cytochrome C oxidase that forms the final reduction of oxygen to water in the respiratory chain. Subunits 1-3 form the functional core. Electrons originating in Cytochrome C are transferred via the copper A center of subunit 2 and the heme A of subunit 1 to the bimetallic center formed by the heme A3 and Copper B. The Cox-1 gene is encoded in the mitochondrion and the protein localizes to the inner mitochondrion membrane. It is expressed in all cells that contain functional mitochondria and mutations in Cox-1 are associated with various mitochondrial dysfunctional diseases that manifest themselves in defective vision, anemia, cardiomyopathy, muscle weakness and even some forms of cancer.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Cox-1 using this Anti-Cox-1 antibody, clone 5C5.1 validated for use in western blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Cox-1 in human thalamus, human kidney and human cerebellum tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Cox-1.
Clone
5C5.1
Concentration
1 mg/mL
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
YP_003024028
Gene Symbol
MT-CO1
COI
COXI
MTCO1
Purification Method
Protein G Purified
UniProt Number
P00395
Molecular Weight
~65 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cox-1 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
