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Millipore This Anti-Ndufs3 Antibody, Clone 6h7.1 Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of Ndufs3. - Mill (Additional S&H or Hazmat Fees May Apply)
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
Complex I-30kD
CI-30kD
NADH-ubiquinone oxidoreductase 30 kDa subunit
Background Information
NDUFS3 is the core subunit of the mitochondrial respiratory chains NADH dehydrogenase complex, also known as Complex I, the complex critical for the production of ATP and NADH electron transfer. In mammals Complex I consists of some 45 different proteins, NDUFS3 is the critical core subunit of the complex. Expressed in all cells and localized in the mitochondria but encoded by a nuclear gene, NDUFS3 plays a critical role in electron transport, our internal cellular respiratory chain, and mitochondrial function. NDUFS3 also is important in disease phenotypes and various cancers. Mutations in NDUFS3 give rise to Leigh syndrome a mitochondrial dysfunctional disease. In cancers, down regulation of NDUFS3 is linked with lowered host survival and increased cancer pathology.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-NDUFS3 Antibody, clone 6H7.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of NDUFS3.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected NDUFS3 in human cerebral cortex, rat hippocampus, and rat cerebellum tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human NDUFS3.
Clone
6H7.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_004542
Gene Symbol
NDUFS3
Purification Method
Protein G Purified
UniProt Number
O75489
Molecular Weight
~30 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in THP-1 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected NDUFS3 in 200 µg of THP-1 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
