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Millipore Anti-Arntl Antibody, Clone 1c5 Is A Highly Specific Mouse Monoclonal Antibody, That Targets Arnt-Like 1 & Has Been Tested In Western Blotting & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
Aryl hydrocarbon receptor nuclear translocator-like protein 1
Basic-helix-loop-helix-PAS protein MOP3
Brain and muscle ARNT-like 1
Class E basic helix-loop-helix protein 5
Short name=bHLHe5
Member of PAS protein 3
PAS domain-containing protein 3
bHLH-PAS protein JAP3
Background Information
Aryl hydrocarbon receptor nuclear translocator-like protein 1 or alternatively, Basic-helix-loop-helix PAS protein MOP3, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5 (bHLHe5), Member of PAS protein 3, PAS domain-containing protein 3, bHLH-PAS protein JAP3, and encoded by the human gene ARNTL,BHLHE5, BMAL1, MOP3 or PASD3, is a protein belonging to the basic helix-loop-helix-PAS domain containing transcription factor family. ARNTL protein is important for the molecular mechanisms behind circadian rhythms as it interacts with a number of CLOCK (Circadian Locomotor Output Cycles Kaput) family members as well as CLOCK itself to promote transcription of a number of proteins of the circadian clock. ARNTL interacts with CRY and CRY2, PER, TIMELESS, and other clock related genes as well as non-CLOCK proteins such as HIF-1alpha (HIF1A) and SUMO3. ARNTL appears critical for proper function of rhythms, as only knockouts of ARNTL in mice generate mice that have upset biological clocks and a shorter lifespan. ARNTL is highly expressed in the adult brain as well as skeletal muscle and heart and ARNTL is a nuclear localized transcription activator. EMD-Millipore’s Anti-ARNTL mouse monoclonal antibody has been tested in western blot against recombinant transfected protein and in paraffin embedded immunohistochemistry on human spleen tissue sections.
Product Information
Format
Ascites
Control
ARNTL-hIgGFc transfected HEK293 cell lysate
Presentation
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-ARNTL Antibody, clone 1C5 is a highly specific mouse monoclonal antibody, that targets ARNT-Like 1 & has been tested in western blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ARNTL in human spleen tissue.
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Purified recombinant fragment of human ARNTL expressed in E. Coli.
Clone
1C5
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
TIC
JAP3
MOP3
BMAL1
PASD3
BMAL1c
bHLHe5
MGC47515
ARNTL
Purification Method
Unpurified
UniProt Number
O00327
Molecular Weight
~100 kDa observed. Uncharacterized bands may appear in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in ARNTL-hIgGFc transfected HEK293 cell lysate.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ARNTL-hIgGFc transfected HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
