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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IHC, IF | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN691 |
| Description | Anti-GPI Antibody, clone 1B7D7 |
| Alternate Names |
|
| Background Information | Glucose-6-phosphate isomerase (GPI) alternatively known as Autocrine motility factor (AMF), Neuroleukin (NLK), Phosphoglucose isomerase (PGI), Phosphohexose isomerase (PHI) or Sperm antigen 36 (SA-36) and encoded by the gene name GPI is major enzyme in glycolysis and gluconeogenesis. Glucose-6-phosphate isomerase rearranges glucose-6-phosphate (from the phosphorylation of glucose via hexokinases) into fructose-6-phosphate (F6P) as the second step in glycolysis. Interestingly however, Glucose-6-phosphate isomerase plays an entirely different sort of role outside of the cell than when it is inside being part of glycolysis. When secreted, Glucose-6-phosphate isomerase acts as an autocrine motility factor for various cancers and is critical for metastasis, while when secreted in nervous tissue it acts as a neurotrophic factor for sensory and spinal neurons and in lymphocytes it acts as a lymphokine upon T cell ligand binding and stimulates B cells to secrete immunoglobulin. Mutations in Glucose-6-phosphate isomerase are the second most frequent cause of inherited glycolytic-enzymopathy in humans. This autosomal recessive disorder is characterized by anon-spherocytic anemia of variable severity which can present with neuromuscular dysfunctions defined by muscle weakness and mental retardation. EMD-Millipore’s Anti-Glucose-6-phosphate isomerase monoclonal antibody has been tested in western blot on recombinant protein as well as cell lysates from HepG2, SMMC-7721 and rat liver tissue and in paraffin embedded immunohistochemistry on human kidney and fluorescent immunocytochemistry on L-O2 cells in culture. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GPI Antibody, clone 1B7D7 is a highly specific mouse monoclonal antibody, that targets Glucose-6-phosphate isomerase & has been tested in western blotting, IHC & Immunofluorescence. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in human kidney tissue. Immunofluorescent Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in L-02 cells. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified recombinant fragment of human GPI expressed in E. Coli. |
| Clone | 1B7D7 |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~56 kDa observed. Uncharacterized bands may appear in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2, SMMC-7721, and rat liver lysates. Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPI in HepG2, SMMC-7721, and rat liver lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |