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Millipore Anti-Wnt10b Antibody, Clone 5a7 Is A Highly Specific Mouse Monoclonal Antibody, That Targets Wnt & Has Been Tested In Ihc, Western Blotting & Immunofluorescence. - Mill (Additional S&H or Hazmat Fees May Apply)
Wingless-type MMTV integration site family member 10B (WNT10B), also known as Protein Wnt-12 (WNT12), consists of structurally related secreted signaling proteins implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is detected in most adult tissues, with the highest levels found in heart and skeletal muscle, and lower levels found in brain. WNT10B may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. Defects in WNT10B are linked to split-hand/foot malformation type 6 (SHFM6).
Product Information
Format
Ascites
Control
HeLa cell lysate
Presentation
Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-WNT10B Antibody, clone 5A7 is a highly specific mouse monoclonal antibody, that targets Wnt & has been tested in IHC, western blotting & Immunofluorescence.
Key Applications
Immunohistochemistry
Western Blotting
Immunofluorescence
Application Notes
Immunohistochemistry Analysis: A 1:500-1,000 dilution from a representative lot detected WNT10B in human kidney and liver tissues.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WNT10B in PANC-1 cells.
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Purified recombinant fragment of human WNT10B expressed in E. coli.
Clone
5A7
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_003385
Gene Symbol
WNY10B
WNT12
Purification Method
Unpurified
UniProt Number
O00744
Molecular Weight
~47 kDa observed. Uncharacterized bands may appear in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WNT10B in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
