Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | ELISA, ICC, Inhibition, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN852-25UG |
| Description | Anti-APP Antibody, clone 2B3 |
| Alternate Names |
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| Background Information | Amyloid-beta A4 protein (UniProt: P05067; also known as ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. APP can also be cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. In addition to its obvious role in Alzheimers disease, the most-substantiated role for APP is in synaptic formation and repair. Its expression is upregulated during neuronal differentiation and after neural injury. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without preservatives. |
| Applications | |
|---|---|
| Application | Anti-APP, clone 2B3, Cat. No. MABN852, is a highly specific mouse monoclonal antibody that targets Amyloid beta A4 protein and has been tested for use in ELISA, Immunocytochemistry, Inhibiton Assay, and Western Blotting. |
| Key Applications |
|
| Application Notes | ELISA Analysis: A representative lot detected APP in ELISA applications (Thomas, R.S., et. Al. (2011). FEBS J. 278(1):167-78; Thomas, R.S., et. Al. (2013). Neuroreport. 24(18):1058-61). Inhibition Analysis: A representative lot inhibited beta amyloid production in MOG-g-UVW culture media (Thomas, R.S., et. Al. (2011). FEBS J. 278(1):167-78). Western Blotting Analysis: A representative lot detected APP in Western Blotting applications (Thomas, R.S., et. Al. (2011). FEBS J. 278(1):167-78). Immunocytochemistry Analysis: A representative lot detected APP in Immunocytochemistry applications (Thomas, R.S., et. Al. (2013). Neuroreport. 24(18):1058-61). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 15 amino acids from the C-terminal region of human Amyloid-beta A4 protein. |
| Epitope | extracellular domain |
| Clone | 2B3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 2B3 specifically detects Amyloid-beta A4 protein in human and murine neurons. It targets an epitope within 15 amino acids including the beta-secretase cleavage site. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rat based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 86.94 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in mouse cortical neurons. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected APP in mouse cortical neurons. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |