Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1180-25UG |
| Description | Anti-E3 ubiquitin-protein ligase UBR1 Antibody, clone 6H9.1 |
| Alternate Names |
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| Background Information | E3 ubiquitin-protein ligase UBR1 (UniProt: Q8IWV7; also known as EC: 2.3.2.27, N-Recognin-1, RING-type E3 ubiquitin transferase UBR1, Ubiquitin-protein ligase E3-alpha-1, Ubiquitin-protein ligase E3-alpha-I) is encoded by the UBR1 gene (Gene ID: 197131) in human. E3 ubiquitin-protein ligase UBR1 is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It may also be involved in pancreatic homeostasis. Two isoforms of UBR1 have been described that are produced by alternative splicing. UBR1 is broadly expressed protein with highest levels reported in skeletal muscle, kidneys, and pancreas. UBR1 has two zinc finger domains. The UBR-type domain is localized to amino acids 97 to 168 and it forms a pocket that mediates recognition of type 1 N-degrons. It exhibits preference for arginine in first position and displays poor affinity for histidine. The second zinc finger domain, RING-type, atypical, is localized to amino acids 1098-1201. Mutations in UBR1 gene are causative factor in Johanson-Blizzard syndrome that is characterized by congenital exocrine pancreatic insufficiency, malformations such as nasal wing aplasia, and mental retardation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-E3 ubiquitin-protein ligase UBR1, clone 6H9.1, Cat. No. MABS1180, is a mouse monoclonal that detects E3 ubiquitin-protein ligase UBR1 and has been tested for use in Immunohistochemistry (Paraffin) Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected E3 ubiquitin-protein ligase UBR1 in human pancreas and human kidney tissue sections. Western Blotting Analysis: 0.5 µg/mL from a representative lot detected E3 ubiquitin-protein ligase UBR1 in C6 cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fragment corresponding to 101 amino acids from the internal region of human E3 ubiquitin-protein ligase UBR1. |
| Clone | 6H9.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 6H9.1 detects E3 ubiquitin-protein ligase UBR1 in human and rat cells. It targets an epitope within 101 amino acids from the internal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~200 kDa observed; 200.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected E3 ubiquitin-protein ligase UBR1 in HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |