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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1198 |
| Description | Anti-SPINK1 Antibody, clone 4D11.1 |
| Alternate Names |
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| Background Information | Serine protease inhibitor Kazal-type 1 (UniProt P00995; also known as Pancreatic secretory trypsin inhibitor, TATI, Tumor-associated trypsin inhibitor) is encoded by the SPINK1 (also known as PCTT, PSTI, TCP) gene (Gene ID 6690) in human. Serine protease inhibitor Kazal-type 1 encoded by the human SPINK1 gene functions as an acute-phase protein (APP) in the pancreas. Produced initially in a 79-a.a. proform, the signal peptide (a.a. 1-23) is removed posttranslationally to yield the 56-a.a. (a.a. 24-79) mature protein. SPINK1 protein blocks premature activation of trypsinogen (inactive form of trypsin), thereby ensuring the integrity of acinar cells to prevent auto-digestion of the pancreas. SPINK1 is also reported to play a role in tissue repair, apoptosis, reproduction, and behave as a growth factor in other human organs. In addition, SPINK1 protein is known as tumor-associated trypsin inhibitor or TATI and current research activities are focused on the association of various cancers to deregulated SPINK1 activity. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SPINK1, clone 4D11.1, Cat. No. MABS1198, is a highly specific mouse monoclonal antibody that targets SPINK1 and has been tested in Immunohistochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SPINK1 in human pancreas tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human SPINK1. |
| Clone | 4D11.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with both pro- and mature human SPINK1. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein g purified. |
| UniProt Number |
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| Molecular Weight | 6.247/8.507 kDa (mature/pro-form) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting of SPINK1 GST fusion protein. Western Blotting Analysis (WB): A 1:5000 dilution of this antibody detected 50 ng of recombinant human SPINK1 GST fusion protein. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |