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Millipore This Anti-Osbp Antibody, Clone 1a6.2 Is Validated For Use In Western Blotting, Immunohistochemistry (Paraffin) For The Detection Of Osbp. - Mill (Additional S&H or Hazmat Fees May Apply)
Oxysterol-binding protein 1 (UniProt P22059) is encoded by the OSBP (also known as OSBP1) gene (Gene ID 5007) in human. OSBP mediates the transport of cholesterol and phosphatidylinositol 4-phosphate (PtdIns4P, PI-4-P, PI4P) between endoplasmic reticulum (ER) and Golgi. OSBP links ER and trans-Golgi membranes at the ER-Golgi membrane contact sites (MCSs) and shuttles sterol into the Golgi and PI4P back to the ER, where it is hydrolyzed by the PI4P-phosphatase Sac1. OSBP contains an FFAT (phenylalanines (FF) in an acidic tract) motif (a.a. 358-364) that binds ER-resident VAP protein and an N-terminal pleckstrin homology (PH) domain (a.a. 88-181) that binds PI4P. OSBP and OSBP-related protein 4 (ORP4) are reported to be involved in virus-induced membrane alterations essential for viral replication organelle formation. OSBP knockdown inhibits virus replication, whereas overexpression of OSBP or ORP4 counteracts the antiviral effects of the OSBP/ORP4 antagonist OSW-1.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-OSBP Antibody, clone 1A6.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of OSBP.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected OSBP in human cerebral cortex, small intestine, and kidney tissue sections.
Biological Information
Immunogen
GST-tagged recombinant human OSBP C-terminal fragment.
Epitope
C-terminal region.
Clone
1A6.2
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 1A6.2 targets an epitope in the C-terminal region of human OSBP.
Isotype
IgG2bκ
Species Reactivity
Human
Species Reactivity Note
Human.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002547
Gene Symbol
OSBP
OSBP1
Purification Method
Protein G purified.
UniProt Number
P22059
Molecular Weight
~90 kDa observed. 89.42 kDa calculated. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected OSBP in 10 µg of Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
