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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1201 |
| Description | Anti-OSBP Antibody, clone 1A6.2 |
| Alternate Names |
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| Background Information | Oxysterol-binding protein 1 (UniProt P22059) is encoded by the OSBP (also known as OSBP1) gene (Gene ID 5007) in human. OSBP mediates the transport of cholesterol and phosphatidylinositol 4-phosphate (PtdIns4P, PI-4-P, PI4P) between endoplasmic reticulum (ER) and Golgi. OSBP links ER and trans-Golgi membranes at the ER-Golgi membrane contact sites (MCSs) and shuttles sterol into the Golgi and PI4P back to the ER, where it is hydrolyzed by the PI4P-phosphatase Sac1. OSBP contains an FFAT (phenylalanines (FF) in an acidic tract) motif (a.a. 358-364) that binds ER-resident VAP protein and an N-terminal pleckstrin homology (pH) domain (a.a. 88-181) that binds PI4P. OSBP and OSBP-related protein 4 (ORP4) are reported to be involved in virus-induced membrane alterations essential for viral replication organelle formation. OSBP knockdown inhibits virus replication, whereas overexpression of OSBP or ORP4 counteracts the antiviral effects of the OSBP/ORP4 antagonist OSW-1. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-OSBP Antibody, clone 1A6.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of OSBP. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected OSBP in human cerebral cortex, small intestine, and kidney tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human OSBP C-terminal fragment. |
| Epitope | C-terminal region. |
| Clone | 1A6.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 1A6.2 targets an epitope in the C-terminal region of human OSBP. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| Purification Method | Protein g purified. |
| UniProt Number |
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| Molecular Weight | ~90 kDa observed. 89.42 kDa calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected OSBP in 10 µg of Jurkat cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |