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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1202 |
| Description | Anti-MKK7/SKK4 Antibody, clone 3F6.5 |
| Alternate Names |
|
| Background Information | Dual specificity mitogen-activated protein kinase kinase 7 (EC 2.7.12.2; UniProt O14733; also known as c-Jun N-terminal kinase kinase 2, MAP kinase kinase 7, MAPK/ERK kinase 7, MAPKK 7, MEK 7, JNK-activating kinase 2, JNK kinase 2, JNKK 2, SAPK kinase 4, SAPKK-4, SAPKK4, Stress-activated protein kinase kinase 4) is encoded by the MAP2K7 (also known as JNKK2, MEK7, MKK7, PRKMK7, SKK4) gene (Gene ID 5609) in human. MKK7 belongs to the family of mitogen-activated protein kinase kinases (MAPKKs) involved in mediating cellular signal transduction in response to proinflammatory cytokines stimulation and environmental stresses. MKK7 and the related MKK4 function as crucial upstream transducers in the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway by phosphorylating different JNK isoforms. MKK7 is activated by upstream MKKKs, whereby the MKKKs interact with MKK7 through its C-terminal DVD domain (a.a. 377-400) and activate MKK7 by phosphorylating serine and threonine residues at a S-K-A-K-T motif located in the MKK7 kinase domain (a.a. 120-380). Upon activation, MKK4 and MKK7 in turn phosphorylate specific tyrosine and threonine residues of the conserved T-P-Y motif in the activation loop of substrate JNK proteins. The N-terminal D domain (a.a. 37-57), consisting of a cluster of positively charged amino acids followed by alternating hydrophobic amino acids, is required for the recruitment of MAPKK substrates, such as JNK. Alternative splicing results in four isoforms. Isoform B (beta), gamma, and isoform 4 differ from the canonical isoform A (alpha) by a 43-a.a. expansion at a.a. 111, a 16-a.a. expansion at a.a. 42, and a 7-a.a. expansion at a.a. 312, respectively, of the MKK7alpha sequence. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-MKK7 Antibody, clone 3F6.5 is validated for use in Western Blotting, Immunocytochemistry for the detection of SKK4. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1 µg/mL from a representative lot detected MKK7/SKK4 in 10 µg of NIH/3T3 cell lysate. Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected MKK7/SKK4 in HeLa, HepG2, and NIH/3T3 cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human MKK7/SKK4 internal fragment. |
| Epitope | Internal (N-terminal half). |
| Clone | 3F6.5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3F6.5 was raised against recombinant N-terminal fragment derived from human MKK7/SKK4 spliced isoform 1 (isoform A or alpha) and isoform 4. The corresponding region in mouse isoform 2 & 8 (isoform alpha & beta 2) differ from the human sequence by only one amino acid (human A69 vs. mouse T69) Cross-reactivity toward other human and murine spliced isoforms is highly expected, but has not been confirmed. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G purified. |
| UniProt Number |
|
| Molecular Weight | ~50 kDa observed. 47.35/51.75/48.94/48.05 kDa (human isoform 1/2/3/4, Met1 removed), 59.18/47.43/42.59/39.25/44.08/49.02/52.37/50.78 kDa (mouse isoform 1/2/3/4/5/6/7/8, Met1 removed) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected MKK7/SKK4 in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |