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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1212 |
| Description | Anti-Geminin Antibody, clone 4B10.1 |
| Alternate Names |
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| Background Information | Geminin (UniProt O75496) is encoded by the GMNN gene (Gene ID 51053) in human. Geminin is a negative regulator of DNA-replication and the founding member of the Geminin family, whose members also include Idas (multicilin; McIdas) and GemC1 (Geminin coiled-coil domain-containing protein 1). Geminin binds Cdt1 and inhibits the mini-chromosome maintenance complex (MCM) loading onto chromatin and pre-replication complex (preRC) formation. Geminin is a 209-amino acid protein that can form homodimer or heterodimers with either Idas or GemC1 via the middle coiled coil structure (a.a. 94-144 of Geminin) in a head-to-tail fashion. The same region in Geminin (a.a. 82-161) is also essential for Cdt1 interaction. A homeodomain-binding domain is located at the C-terminal region of Geminin (a.a. 170-190). GemC1 is also implicated in DNA replication but at a later stage than Geminin. All three Geminin family members play important roles in cell proliferation and differentiation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Geminin using this mouse monoclonal Anti-Geminin, clone 4B10.1 Antibody, Cat. No. MABS1212, validated for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected nuclear geminin immunoreactivity in HUVECs, A431, and HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human geminin internal fragment. |
| Clone | 4B10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4B10.1 detects human geminin by targeting an internal epitope. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified. |
| UniProt Number |
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| Molecular Weight | ~31 kDa observed. 23.57 kDa calculated. The larger-than-predicted band size is consistent with that reported in the literature (McGarry, T.J., and Kirschner, M.W. (1998). Cell. 93(6):1043-1053 ). Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected geminin in 10 µg of HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |