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Millipore Anti-T4 Lysozyme (T4l) Antibody, Clone 6a6, Cat. No. Mabs1289, Is A Highly Specific Mouse Monoclonal Antibody That Targets Bacteriophage T4 Lysozyme (T4l), Validated For - Mill (Additional S&H or Hazmat Fees May Apply)
Bacteriophage T4 endolysin (EC 3.2.1.17; UniProt P00720; also known as T4 lysis protein, T4 lysozyme, T4 muramidase, T4L) is encoded by the T4 E gene. T4 lysozyme (T4L) is a 164-a.a. monomeric protein that hydrolyzes peptidoglycan.T4L functions outside the cytoplasmic membrane and starts the phage extracellular phase by liberating virions from the metabolically inert remains of the bacterial host. Similar to glutathione S-transferase (GST) and T7 lysozyme, T4L sequence is commonly utilized to generate soluble recombinant fusions of hydrophobic proteins, such as multitransmembrane proteins, that are otherwise not soluble when expressed entirely in their native sequences. Antibodies against T4L are useful in detecting T4L in T4-infected bacteria hosts and in probing recombinant T4L fusion proteins.
Product Information
Format
Purified
Presentation
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-T4 Lysozyme (T4L) Antibody, clone 6A6, Cat. No. MABS1289, is a highly specific mouse monoclonal antibody that targets bacteriophage T4 lysozyme (T4L), validated for use in Immunoprecipitation and Western Blotting for the detection of T4L and T4L-tagged fusion proteins.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated exogenously expressed TL4 fusion protein from 1.5 µg of lysate of transfected cells by an independent laboratory.
Biological Information
Immunogen
Recombinant T4L fusion protein.
Clone
6A6
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 6A6 detects T4L and recombinant T4L fusion proteins.
Isotype
IgG1κ
Species Reactivity
All
Species Reactivity Note
Bacteriophage T4.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_049736.1
Gene Symbol
Bacteriophage T4 gene E
Purification Method
Protein G purified.
UniProt Number
P00720
Molecular Weight
18.69 kDa calculated.
Product Usage Statements
Quality Assurance
Western Blotting Analysis: 0.1 µg/mL of this antibody detected exogenously expressed T4L fusion protein in 0.2 µg of lysate from transfected cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
