Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, ELISA, ICC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1310-25UL |
| Description | Anti-Apolipoprotein A-I Antibody, clone Wt20-7 |
| Alternate Names |
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| Background Information | Apolipoprotein A-I (UniProt: P02647; also known as Apo-AI, ApoA-I, Apolipoprotein A1) is encoded by the APOA1 gene (Gene ID: 335) in human. Apo A-1 is a major protein of plasma high density lipoprotein (HDL) and is also found in chylomicrons. It plays a major role in transporting cholesterol from peripheral tissues to the liver. It is synthesized mainly in the liver with a signal peptide (aa 1-18), which is proteolytically cleaved to generate the mature protein. Apo A-1 also serves as a co-factor for lecithin cholesterolacyltransferase (LCAT) that is involved in the formation of cholesteryl esters. Elevated levels of oxidized Apo A-1 (on Methionine 110 and 136) have been linked to increased risk for coronary artery disease. Mutations in APOA1 gene have also been linked to HDL deficiencies that lead to premature coronary artery disease and a reduction in cellular cholesterol efflux. Mutations in APOA1 gene have also shown to result in amyloidosis with extensive visceral amyloid deposit and in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as familial amyloid polyneuropathy type III. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Apolipoprotein A-I, clone Wt20-7, Cat. No. MABS1310, is a mouse monoclonal antibody that detects human Apolipoprotein A-I and has been tested for use in DOT Blot, ELISA, Immunocytochemistry, and Western Blotting. |
| Key Applications |
|
| Application Notes | ELISA Analysis: A representative lot detected Apolipoprotein A-I in ELISA applications (Kimura, H., et. Al. (2017). Sci Rep. 7(1):2988). Western Blotting Analysis: A representative lot detected Apolipoprotein A-I in Western Blotting applications (Kuwabara, K., et. Al. (2015). J Biol Chem. 290(40):24210-21). Immunocytochemistry Analysis: A representative lot detected Apolipoprotein A-I in Immunocytochemistry applications (Kameyama, H., et. Al. (2016). Sci Rep. 6:30391; Kuwabara, K., et. Al. (2015). J Biol Chem. 290(40):24210-21). DOT Blot Analysis: A representative lot detected Apolipoprotein A-I in DOT Blot applications (Kimura, H., et. Al. (2017). Sci Rep. 7(1):2988; Kameyama, H., et. Al. (2016). Sci Rep. 6:30391; Kuwabara, K., et. Al. (2015). J Biol Chem. 290(40):24210-21). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged full-length human recombinant Apolipoprotein A-I |
| Clone | Wt20-7 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone Wt20-7 specifically detects human Apolipoprotein A-I. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~28 kDa observed; 30.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human fetal liver tissue lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apolipoprotein A-I in human fetal liver tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |