Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | Function Assay, IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1320-25UL |
| Description | Anti-Serpin B13 Antibody, clone B29 |
| Background Information | Serpin B13 (UniProt: Q8CDC0) is encoded by the Serpinb13 gene (Gene ID: 241196) in murine species. Serpin B13 is a cytoplasmic protein that plays a role in the proliferation or differentiation of keratinocytes. Two isoforms of Serpin B13 have been described that are generated by alternative splicing. Serpin B13 acts as a serine proteinase inhibitor and studies have shown a strong induction of serpin B13 in the pancreas of mice during the first few weeks of life. Cathepsin L and K appear to be the targets of serpin B13 action. Serpin B13 is expressed in pancreas with low levels of expression in pancreatic islets and larger expression in the exocrine pancreas. Secretion of anti-serpin B13 autoantibodies in young diabetes-prone non-obese diabetic (NOD) mice is shown to be associated with reduced inflammation in pancreatic islets and a slower progression to autoimmune diabetes. Anti-serpin B13 autoantibodies are shown to protect NOD mice against diabetes before 16 weeks of age. Reduced expression of serpin B13 autoantibodies is associated with development of type I diabetes in human before 5 years of age. Mice injected with monoclonal anti-serpin B13 display few inflammatory cells in the islets and rapid recovery from recent onset diabetes. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Serpin B13, clone B29, Cat. No. MABS1320, is a mouse monoclonal antibody that detects Serpin B13 and has been tested for use in Function Studies, Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A representative lot detected Serpin B13 in Immunofluorescence applications (Baldzizhar, R., et. Al. (2012). J Biol Chem. 288(3):1612-9). Western Blotting Analysis: 3 ug/mL from a representative lot detected Serpin B13 in mouse serpin/HEK293 cell lysates (Courtesy of Dr. Jan Czyzyk, M.D., University of Rochester Medical Center, NY). Western Blotting Analysis: A representative lot detected Serpin B13 in Western Blotting applications (Baldzizhar, R., et. Al. (2012). J Biol Chem. 288(3):1612-9; Czyzyk, J., et. Al. (2012). J Immunol. 188(12):6319-27). Affects Function Analysis: A representative lot detected Serpin B13 in Affects Function applications (Baldzizhar, R., et. Al. (2012). J Biol Chem. 288(3):1612-9). |
| Biological Information | |
|---|---|
| Immunogen | Purified full length mouse Serpin B13 resuspended in Complete Freunds Adjuvant. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone B29 detects Serpin B13 in human and murine species. |
| Isotype | IgG2b |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
|
| Molecular Weight | 44.32 kda calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Isotype testing: Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1κ. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |