Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1814-25UL |
| Description | Anti-GATA2 Antibody, clone 3C10.1 |
| Alternate Names |
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| Background Information | Endothelial transcription factor GATA-2 (UniProt: P23769; also known as GATA-binding protein 2) is encoded by the GATA2 gene (Gene ID: 2624) in human. GATA2 is a zinc-finger transcriptional activator that regulates endothelin-1 gene expression in endothelial cells. It binds to the consensus sequence 5-AGATAG-3. GATA2 is expressed in embryonic brain and liver, endothelial cells, and in erythroid cells. GATA2 mutations appear to cause loss of function of the mutated allele leading to haploinsufficiency, which results in immunodeficiency characterized by significantly reduced or absent monocytes, B-lymphocytes, natural killer lymphocytes, and circulating and tissue dendritic cells. However, it does not affect the T-cell number. This deficiency leads to increased susceptibility to mycobacterial, papillomavirus, and opportunistic fungal infections. Certain mutations in GATA2 are also linked to lymphedema that results in swelling of the extremities due to altered lymphatic flow. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-GATA2 Antibody, clone 3C10.1, Cat. No. MABS1814, is a mouse monoclonal antibody that targets GATA2 and has been tested in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fragment corresponding to 114 amino acids from the internal region of human GATA2. |
| Clone | 3C10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3C10.1 specifically detects Endothelial transcription factor GATA-2 in human. It targets an epitope within 114 amino acids from the internal region. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Mouse based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~52 kDa observed; 50.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GATA2 in 10 µg of SH-SY5Y cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |