Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, FC, ICC, IH(P), IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1911-25UL |
| Description | Anti-Serpin B9 Antibody, clone 7D8 |
| Alternate Names |
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| Background Information | Serpin B9 (UniProt: P50453; also known as Cytoplasmic antiproteinase 3, CAP-3, CAP3, Peptidase inhibitor 9, PI-9) is encoded by the SERPINB9 (also known as PI9) gene (Gene ID: 5272) in human. Serpins serve as intracellular or extracellular regulators of a wide range of physiological processes such as complement activation, blood coagulation and apoptosis. Within the vertebrate immune system, they control proteases of the classical and innate complement systems, and are also potent inhibitors of many leukocyte granule proteases. Serpin B9 has a broad tissue distribution, being present at high and relatively stable levels natural killer cells and CD8+ T cells, antigen-presenting cells, in many endothelial and mesothelial cells and at sites of immune privilege such as testis and placenta. Serpin B9 serves as a regulator granzyme B activity by acting as an inhibitor of its activity. Overexpression of Serpin B9 may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. Certain tumors may upregulate Serpin B9 levels as a mechanism of immune evasion. (Ref.: Kaiserman, D., and Bird, PI (2010). Cell Death Differen. 17(4); 586-595). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Serpin B9, clone 7D8, Cat. No. MABS1911, is a mouse monoclonal antibody that detects Serpin B9 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | ELISA Analysis: A representative lot detected Serpin B9 in ELISA applications (Hirst, C.E., et. Al. (2001). Mol Hum Peprod. 7(12):1133-42). Flow Cytometry Analysis: A representative lot detected Serpin B9 in Flow Cytometry applications (Hirst, C.E., et. Al. (2003). J Immunol. 170(2):805-15). Immunocytochemistry Analysis: A representative lot detected Serpin B9 in Immunocytochemistry applications (Hirst, C.E., et. Al. (2003). J Immunol. 170(2):805-15). Immunofluorescence Analysis: A representative lot detected Serpin B9 in Immunofluorescence applications (Hirst, C.E., et. Al. (2003). J Immunol. 170(2):805-15). Immunohistochemistry Analysis: A representative lot detected Serpin B9 in Immunohistochemistry applications (Hirst, C.E., et. Al. (2001). Mol Hum Peprod. 7(12):1133-42). Western Blotting Analysis: A 1:100 dilution from a representative lot detected Serpin B9 in YT and Jurkat cell lysates (Courtesy of Prof. Phillip Bird, pH.D., Monash University, Victoria Australia). Western Blotting Analysis: A representative lot detected Serpin B9 in Western Blotting applications (Hirst, C.E., et. Al. (2001). Mol Hum Peprod. 7(12):1133-42; Hirst, C.E., et. Al. (2003). J Immunol. 170(2):805-15; Mangan, M.S., et. Al. (2015). J Biol Chem. 291(7):3626-38). Immunocytochemistry Analysis: A representative lot detected Serpin B9 in COS-1 cells (Courtesy of Prof. Phillip Bird, pH.D., Monash University, Victoria Australia). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full length human recombinant Serpin B9. |
| Clone | 7D8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 7D8 detects Serpin B9 in human cells. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 42.40 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human spleen tissue sections. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Serpin B9 in human spleen tissue sections. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |