Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ELISA, IHC, FC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1915-25UG |
| Description | Anti-EGFRvIII Antibody, clone DH8.3 |
| Alternate Names |
|
| Background Information | Epidermal growth factor receptor (UniProt: P00533; also known as EC:2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is a single-pass type 1 membrane protein that is ubiquitously expressed. It is synthesized with a 24 amino acids signal peptide that is subsequently cleaved. It contains an extracellular domain (aa 25-645) a transmembrane domain (aa 646-668), and a cytoplasmic domain (aa 669-1210). Upon binding of EGF, the receptor is phosphorylated and recruits adapter proteins to turn on downstream signaling cascades. EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes. The most common truncated EGFR is the variant III EGFR deletion mutant (EGFRvIII), containing an inframe deletion of exons 2 7 (801 bp) from the extracellular domain. EGFRvIII mutation is reported in about 5% of human lung squamous cell carcinoma (SCC). It has also been suggested that EGFRvIII potentially plays an essential role in the human NSCLC tumor initiation and maintenance. Drugs such as Gefitinib, erlotnib, and HKI-272 are shown to inhibit the growth of EGFRvIII transformed cells and tumor growth. (Ref.: Ji, H., et Al. (2006). Proc. Natl. Acad. Sci. USA 103(20); 7817-7822). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti–EGFRvIII, clone DH8.3 Antibody, Cat. No. MABS1915-25UG, validated for use in Western Blotting, ELISA, Immunohistochemistry, Flow Cytometry and Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected EGFRvIII in Western Blotting applications (Lammering, g., et. Al. (2004). Clin Cancer Res. 10(19):6732-43; Nishikawa, R., et. Al. (2004). Brain Tumor Pathol. 21(2):53-6; Hills, D., et. Al. (1995). Int J Cancer. 63(4):537-43). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to EGFRvIII (EGFR variant III) (LEEKKGNYVVTDHC) sequence. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone DH8.3 detects EGFR variant vIII a truncated form of EGFR, present in human cancer cells. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in U87MG.∆EGFR cell lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |