| Description | |
|---|---|
| Catalogue Number | MABS1963-25UG |
| Description | Anti-PEG Antibody, clone AGP3 |
| Alternate Names |
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| Background Information | Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. Clone AGP3 is a mouse monoclonal IgM antibody that displays high affinity to both short and long PEG molecules. It displays specificity for the repeating subunits present in PEG and can bind a broad range of PEG molecules. It is suitable for use as a capture antibody in ELISA with biotin-conjugated anti-PEG, clone 6.3 (Cat. No. MABS1966) as a detection reagent. Use of CHAPS detergent instead of Tween-20 is highly recommended in all washing buffers. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-PEG, clone AGP3, Cat. No. MABS1963, is a mouse monoclonal antibody that detects Polyethylene glycol (PEG) and has been tested for use in ELISA, Immunohistochemistry, and Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A represenative lot detected PEG in Western Blotting applications (Cheng, T.L., et. Al. (2005). Bioconjug Chem. 16(5):1225-31). ELISA Analysis: A represenative lot detected PEG in ELISA applications (Cheng, T.L., et. Al. (2005). Bioconjug Chem. 16(5):1225-31). Immunohistochemistry Analysis: A represenative lot detected PEG in Immunohistochemistry applications (Ton, N.C., et. Al. (2007). Clin Cancer Res. 13(23):7113-8). |
| Biological Information | |
|---|---|
| Immunogen | PEG-conjugated to E.coli beta-glucuronidase. |
| Clone | AGP3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone AGP3 is a mouse monoclonal antibody with specificity for polyehtylene glycol backbone. |
| Isotype | IgMκ |
| Species Reactivity |
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| Species Reactivity Note | All. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Purified by ion-exchange chromatography. |
| Molecular Weight | N/A |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by ELISA with immobilized Polyethylene Glycol (10K-PEG, 3K-PEG, and 2K-mPEG). ELISA Analysis: 20 µg/mL of Polyethylene Glycol (PEG) (10K-PEG, 3K-PEG, and 2K-mPEG) were detected by this antibody. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |