| Description | |
|---|---|
| Catalogue Number | MABS1964-25UG |
| Description | Anti-PEG Antibody, clone AGP4 |
| Alternate Names |
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| Background Information | Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. PEG is considered as a non-immunogenic moiety and has been widely tested for its ability to protect proteins from the host s immune system. This antibody (Clone AGP4) binds to the PEG backbone and is suitable for capture of PEGylated compounds in ELISA as well as the detection of PEGylated molecules. It is suitable for sensitive ELISA applications where AGP4 can be used as a capture antibody. Note: Tween-20 should be used in wash buffers for this antibody combination. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-PEG, clone AGP4, Cat. No. MABS1964, is a mouse monoclonal antibody that detects Polyethylene glycol (PEG) and has been tested for use in ELISA, Western Blotting, and Immunohistochemistry. |
| Key Applications |
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| Application Notes | Immunohistochemistry Analysis: A representative lot detected PEG in Immunohistochemistry applications (Wang, H., et. Al. (2012). J Virol. 86(11):6286-302). Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, g.K., et. Al. (2009). J Mol Recognit. 22(2):99-103). ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et Al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. Al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. Al. (2016). Acta Biomater. 43:61-70). |
| Biological Information | |
|---|---|
| Immunogen | Polyethylene glycol-conjugated to E.coli beta-glucuronidase. |
| Clone | AGP4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone AGP4 specifically detects proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs). |
| Isotype | IgMκ |
| Species Reactivity |
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| Species Reactivity Note | All. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Purified by ion-exchange chromatography |
| Molecular Weight | N/A |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by ELISA with amino- and methoxy PEG molecules (10K-PEG, 3K-PEG, and 2K-mPEG). ELISA Analysis: 5 µg/mL of this antibody detected amino- and methoxy-PEG molecules (10K amino-PEG, 3K-amino PEG, and 2K-methoxy PEG). |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |