Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, FC, IH(P), Inhibition, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1976-25UG |
| Description | Anti-Del-1 Antibody, clone D345 |
| Alternate Names |
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| Background Information | EGF-like repeat and discoidin I-like domain-containing protein 3 (UniProt: O43854; also known as Developmentally-regulated endothelial cell locus 1 protein, Integrin-binding protein DEL1, Del-1) is encoded by the EDIL3 (also known as DEL1) gene (Gene ID: 10085) in human. Del-1 is a calcium-dependent glycoprotein that is secreted by endothelial cells and is shown to be expressed in a developmentally restricted pattern during embryogenesis. In adult tissues it is expressed in endothelial cells and in macrophages. Del-1 is reported to prevent apoptosis in endothelial cell subjected to TNF alpha, etoposide, and anoikis treatment. This anti-apoptotic effect is shown to be mediated specifically through binding of integrin alphav beta3 by the RGD motif. Del-1 expression is reported to be inversely related to that of IL-17 that is shown to inhibit Del-1 via a GSK-3beta and C/EBPb-dependent mechanism. This inhibitory action can be reversed at the PI3K/Akt signaling induced by D-resolvins. It serves as an endothelial cell-secreted homeostatic factor that regulates local inflammation in several tissues. It is reported to interfere with beta2-integrin-dependent inflammatory cell adhesion to endothelium and restricts transendothelial migration of neutrophils. Del-1 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off in the mature form. It has three EGF-like domains in its N-terminal region. EGF-like domains 2 and 3 form a rigid rod via an interdomain calcium ion binding site, while the long linker between EGF1 and EGF2 lends considerable flexibility to EGF1. Del-1 also has discoidin I-like domains at the C-terminal region that mediate its binding to phosphatidylserine. Two isoforms of Del-1 have been described that are produced by alternative splicing. (Ref.: Dasgupta, SK et Al. (2012). Circulation 125 (13); 1664-1672; Wang, Z., et Al. (2012). Surgery 151(2); 296-305; Maekawa, T et Al. (2015). Nat. Commun. DOI: 10.1038/ncomms9272). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide. |
| Applications | |
|---|---|
| Application | Detects Del-1 using this Anti-Del-1, clone D345, Cat. No. MABS1976, is a mouse monoclonal antibody that detects Developmentally-regulated endothelial cell locus 1 protein (Del-1) and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition assay, & Western Blot. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Del-1 in human cerebral cortex tissue sections. ELISA Analysis: A representative lot detected Del-1 in ELISA applications (Dasgupta, S.K., et. Al. (2012). Ciculation. 125(13):1664-72; Maekawa, T., et. Al. (2015). Nat Commun. 6:8272). Inhibition Analysis: A representative lot inhibited the uptake of BODIPY-labeled platelet microparticles by endothelium. (Dasgupta, S.K., et. Al. (2012). Ciculation. 125(13):1664-72). Western Blotting Analysis: A representative lot detected Del-1 in Western Blotting applications (Dasgupta, S.K., et. Al. (2012). Ciculation. 125(13):1664-72). Flow Cytometry Analysis: A representative lot detected Del-1 in Flow Cytometry applications (Dasgupta, S.K., et. Al. (2012). Ciculation. 125(13):1664-72). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged full length human recombinant Developmentally-regulated endothelial cell locus 1 protein (Del-1). |
| Clone | D345 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone D345 detects human Developmentally-regulated endothelial cell locus 1 protein (Del-1). |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 53.76 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in human pancreas tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Del-1 in human pancreas tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |