| Description | |
|---|---|
| Catalogue Number | MABS2000 |
| Description | Anti-LXR alpha Antibody, clone 2B7 |
| Alternate Names |
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| Background Information | Oxysterols receptor LXR-alpha (UniProt: Q62685; also known as Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3, RLD-1) is encoded by the Nr1h3 (also known as Lxra) gene in rat. Liver X receptors (LXRs) are members of the nuclear receptor superfamily that have been implicated in lipid homeostasis and glucose metabolism. Oxysterols, oxidized derivatives of cholesterol, are ligands for LXRs. LXRs act as cholesterol sensors; when cellular oxysterols accumulate as a result of increasing concentrations of cholesterol, LXR induces the transcription of genes that protect cells from cholesterol overload. LXRs bind to consensus elements (LXR response elements, LXREs) as heterodimers with isoforms of the retinoid X receptor (RXR). LXR-alpha is an important regulator of cholesterol and bile acid metabolism. It interacts with RXR and shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. In adults it is expressed in spleen, pituitary, lung, liver, and fat and a weaker expression is observed in several other tissues. LXR-alpha contains a transactivation domain (AF-1; aa 1-94) that is required for ligand-independent transactivation function; another transactivation domain (AF-2; aa 203-445) that is required for ligand-dependent transactivation function; and a ligand binding domain (aa 213-432). (Ref.: Zhao, C., and Dahlman-Wright, K. (2010). J. Endocrinol. 204, 233-240). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LXR alpha, clone 2B7, Cat. No. MABS2000, is a mouse monocloanl antibody that detects Oxysterols receptor LXR-alpha and has been tested for use in Immunoprecipitation and Western Blotting. |
| Key Applications |
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| Application Notes | Immunoprecipitation Analysis: A representative lot detected LXR alpha in Immunoprecipitation applications (Tian, J., et. Al. (2016). Proc Natl Acad Sci USA. 113(29):8182-7). Western Blotting Analysis: A representative lot detected LXR alpha in Western Blotting applications (Tian, J., et. Al. (2016). Proc Natl Acad Sci USA. 113(29):8182-7). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged full length recombinant rat LXR-alpha protein. |
| Clone | 2B7 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 2B7 detects LXR-alpha in mouse and rat liver tissue. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~50 kDa observed; 50.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in mouse liver tissue lysate. Western Blotting Analysis: 1 ug/mL of this antibody detected LXR alpha in 10 µg of mouse liver tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 100 μg |