| Description | |
|---|---|
| Catalogue Number | MABS2002-25UG |
| Description | Anti-mPEG Antibody, clone 15-2b |
| Alternate Names |
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| Background Information | Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solutions. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. The monomethoxylated form of polyethylene glycol (mPEG) has been widely employed in drug modifications. mPEG modification reduces nonspecific binding and prevents recognition by the reticuloendothelial system. This monoclonal antibody (clone 15-2b) specifically binds to the terminal methoxy group, but not the backbone of PEG. It displays high affinity for the terminal methoxy group and can be easily used as a detection antibody for the quantification of mPEG by ELISA. It is also suitable for detection of PEGylated liposomes and nanoparticles. The mPEG cell-based competition ELISA can detect lower molecular-weight (< 2 kDa) mPEG molecules even at nanomolar concentration and the assay is not affected by the presence of mouse or human serum. In addition, mPEG cell-based ELISA can be useful in determining the pharmacokinetics of a mPEG molecule an animal without the need for any radiolabeling. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in PBS with 0.04% sodium azide. |
| Applications | |
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| Application | Anti-mPEG, clone 15-2b, Cat. No. MABS2002, is a mouse monoclonal antibody that detects methoxy-PEG (mPEG)-conjugated proteins and has been tested for use in ELISA. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Methoxy PEG covalently conjugated to E.coli beta-glucuronidase. |
| Clone | 15-2b |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 15-2b specifically detects proteins conjugated with methoxy polyethylene glycol (mPEG). |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | All. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein A purified |
| Molecular Weight | N/A |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by ELISA with methoxy Polyethylene Glycol (10KPEG, 3KPEG, and 2K mPEG). ELISA Analysis: 5 µg/mL of this antibody detected methoxy polyethylene glycol (2K-mPEG) and with minimal reactivity with 10K and 3K amino-PEGs. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |